高复杂度空间分析与CODEX：生物信息学分析及其在人类疾病中的应用。
Highly multiplexed spatial profiling with CODEX: bioinformatic analysis and application in human disease.
发表日期：2023 Jan
作者：
Wilson Kuswanto, Garry Nolan, Guolan Lu
来源：
Seminars in Immunopathology
摘要：
多重成像技术允许我们在组织内对蛋白质和RNA进行空间定位，是现有多组学技术的有益补充，并深化了我们对健康和疾病的理解。CODEX作为一种多重单细胞成像技术，采用了微流控系统，并结合DNA条形码抗体，可以在单细胞水平上显示50个以上的细胞标记。在本文中，我们讨论了CODEX在癌症、自身免疫和感染研究中的最新应用，以及从预处理到细胞分割和标记量化到空间分析技术的当前生物信息学方法来分析多重成像数据。最后，我们就多重空间分析面临的挑战和未来发展进行评论。© 2022. The Author(s).
Multiplexed imaging, which enables spatial localization of proteins and RNA to cells within tissues, complements existing multi-omic technologies and has deepened our understanding of health and disease. CODEX, a multiplexed single-cell imaging technology, utilizes a microfluidics system that incorporates DNA barcoded antibodies to visualize 50 + cellular markers at the single-cell level. Here, we discuss the latest applications of CODEX to studies of cancer, autoimmunity, and infection as well as current bioinformatics approaches for analysis of multiplexed imaging data from preprocessing to cell segmentation and marker quantification to spatial analysis techniques. We conclude with a commentary on the challenges and future developments for multiplexed spatial profiling.© 2022. The Author(s).