本研究的目的是评估Bdellovibrio bacteriovorus HD100对实验性牙周炎（EP）大鼠的影响。32只大鼠被分为四组：对照组、C-HD100组（B. bacteriovorus）、EP组和EP-HD100组。在第0天，将棉线环绕在下颌第一颗磨牙（MFMs）周围诱导EP和EP-HD100组。在C-HD100和EP-HD100组中，在第0、3和7天向MFMs亚龈区域中局部赋予含1×109 PUF/ml的B. bacteriovorus HD100的悬浮液。在第14天动物被安乐死。在半面下颌骨中进行形态计量学分析。通过酶联免疫吸附测定在龈组织中测定肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-6、单核细胞趋化蛋白（MCP）-1、IL-10、IL-1β、转化生长因子β（TGF-β）、巨噬细胞集落刺激因子（M-CSF）和规管激活和正常T细胞表达和分泌的核酸（RANTES）。通过免疫组化分析BD-1、BD-2和BD-3，Toll样受体（TLR）-2和TLR-4以及分化聚集（CD）-4、CD-8和CD-57的表达情况。数据进行了统计分析。EP组显示比EP-HD100更大的牙槽骨损失（p <.05）。EP-HD100组显示比EP组MCP-1、RANTES、IL-10和TGF-β水平更高、TNF-α水平更低（p <.05）。EP组和EP-HD100组之间的IL-1β、IL-6和M-CSF水平没有差异。C-HD100组的IL-6、TNF-α、RANTES和MCP-1水平高于对照组（p <.05）。关于BD，EP-HD100组显示比EP组更大的BD-1、BD-2和BD-3免疫标记模式（p <.05）。EP组和EP-HD100组之间的TLR-2、TLR-4、CD-4、CD-8和CD-57的免疫标记模式没有显着差异。B. bacteriovorus HD100的局部应用降低了鼠EP时的牙槽骨损失、增加了BD的表达，并调节了牙周组织中的细胞因子水平。

The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats.Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by the placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 109  PUF/ml of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analyses were performed in hemimandibles. The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, transforming growth factor beta (TGF-β), macrophage colony-stimulating factor (M-CSF) and regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta defensin (BD)-1, BD-2, and BD-3, Toll-like receptors (TLR)-2 and TLR-4, and a cluster of differentiation (CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed.The EP group showed greater alveolar bone loss than EP-HD100 (p < .05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p < .05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES, and MCP-1 levels than the control group (p < .05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p < .05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD-4, CD-8, and CD-57 between EP and EP-HD100 groups.The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of BD, and modulates the cytokines levels on periodontal tissues in rats with EP.© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.