全球范围内，口腔鳞状细胞癌（OSCC）是最常见的致命疾病之一。其高死亡率归因于该疾病通常在晚期被诊断，这表明急需寻找早期检测OSCC的方法。使用唾液自身抗体（autoAbs）作为OSCC生物标志物有许多优点，例如易于获得唾液样本，并且利用成熟的二级试剂高效检测autoAbs。为了改善OSCC筛查，我们结合亲和质谱（MS）技术，通过富集唾液自身抗体来鉴定与OSCC相关的自身抗体。将健康个体和OSCC患者的唾液IgA用肽M偶联珠纯化，然后应用于OSCC细胞中免疫沉淀的抗原（Ags）。使用串联质谱分析和基于光谱计数的定量分析，10个抗原在OSCC组中的水平显著高于对照组。此外，通过多重串珠法测定了自身抗体对这10个抗原的唾液水平。其中，有七个在早期OSCC患者中显著高于健康个体。为了改善OSCC的早期诊断，进一步制定了由对LMAN2、PTGR1、RAB13和UQCRC2的自身抗体构成的标记板。© 2023年Wiley-VCH GmbH。

Globally, oral cavity squamous cell carcinoma (OSCC) is one of the most common fatal illnesses. Its high mortality is ascribed to the fact that the disease is often diagnosed at a late stage, which indicates an urgent need for approaches for the early detection of OSCC. The use of salivary autoantibodies (autoAbs) as OSCC biomarkers has numerous advantages such as easy access to saliva samples and efficient detection of autoAbs using well-established secondary reagents. To improve OSCC screening, we identified OSCC-associated autoAbs with the enrichment of salivary autoAbs combined with affinity mass spectrometry (MS). The salivary IgA of healthy individuals and OSCC patients was purified with peptide M-conjugated beads and then applied to immunoprecipitated antigens (Ags) in OSCC cells. Using tandem MS analysis and spectral counting-based quantitation, the level of 10 Ags increased in the OSCC group compared with the control group. Moreover, salivary levels of autoAbs to the 10 Ags were determined by a multiplexed bead-based immunoassay. Among them, seven were significantly higher in early-stage OSCC patients than in healthy individuals. A marker panel consisting of autoAbs to LMAN2, PTGR1, RAB13, and UQCRC2 was further developed to improve the early diagnosis of OSCC.© 2023 Wiley-VCH GmbH.