新出现的报告表明，长非编码RNA（lncRNA）在癌症的发病和转移中发挥作用。然而，LncRNA CEBPA-AS1在急性髓样白血病（AML）中的生物功能和潜在机制仍然不明确。利用荧光原位杂交（FISH）和逆转录定量聚合酶链反应（RT-qPCR）检测AML临床组织和细胞系中CEBPA-AS1的水平。应用体内和体外功能测试以鉴定CEBPA-AS1在AML发展中的增癌作用。过表达的CEBPA-AS1与AML患者的不良生存率有关。此外，生物信息学预测并通过RNA免疫共沉淀（RIP）和荧光素酶报告基因实验验证了CEBPA-AS1、锌指蛋白X-连锁（ZFX）和miR-24-3p之间的关系。我们的发现揭示了转录因子ZFX特别相互作用于CEBPA-AS1启动子并激活CEBPA-AS1转录。CEBPA-AS1的下调抑制了AML细胞的增殖和侵袭，同时促进细胞凋亡，在体内和体外的异种移植瘤生长中有变化。然而，CEBPA-AS1的过表达观察到相反的效果。此外，CEBPA-AS1作为miR-24-3p的竞争性内源性RNA（ceRNA），以减弱miR-24-3p对其下游靶点CTBP2的抑制作用。总的来说，这项研究强调了CEBPA-AS1在AML中的增癌作用，并阐明了它与上游转录因子ZFX和下游调节轴miR-24-3p/CTBP2的联系，为AML中的癌症过程提供了重要见解。©2023年。作者（们）在Springer Nature B.V.拥有独家许可。

Emerging reports demonstrated that long non-coding RNAs (lncRNAs) play a role in the pathogenesis and metastasis of cancers. However, the biological functions and underlying mechanisms of LncRNA CEBPA-AS1 in acute myeloid leukemia (AML) remain largely elusive. The level of CEBPA-AS1 was examined in AML clinical tissues and cell lines via fluorescence in situ hybridization (FISH) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). In vivo and in vitro functional tests were applied to identify the pro-oncogenic role of CEBPA-AS1 in AML development. The overexpressed CEBPA-AS1 was linked to poor survival in AML patients. Moreover, the relationships among CEBPA-AS1, Zinc Finger Protein X-Linked (ZFX), and miR-24-3p were predicted by bioinformatics and validated by RNA immunoprecipitation (RIP) and luciferase reporter assays. Our findings unveiled that transcription factor ZFX particularly interacted with the promoter of CEBPA-AS1 and activated CEBPA-AS1 transcription. Downregulation of CEBPA-AS1 inhibited the proliferation and invasion while promoted apoptosis of AML cells in in vitro, as well as in vivo, xenograft tumor growth was modified. However, overexpression of CEBPA-AS1 observed the opposite effects. Furthermore, CEBPA-AS1 acted as a competitive endogenous RNA (ceRNA) of miR-24-3p to attenuate the repressive effects of miR-24-3p on its downstream target CTBP2. Taken together, this study emphasized the pro-oncogenic role of CEBPA-AS1 in AML and illustrated its connections with the upstream transcription factor ZFX and the downstream regulative axis miR-24-3p/CTBP2, providing important insights to the cancerogenic process in AML.© 2023. The Author(s), under exclusive licence to Springer Nature B.V.