强效预防和治疗复合物的工程一直需要仔细的蛋白质修饰技术，并保持无缝能力。在这种情况下，支持无阻多价靶向和正确抗原呈现的方法仍然是必不可少且非常苛刻的。多年来，Sortase A（SrtA）跨肽反应表现出这些特征。但是，特别是在当前需要积极靶向和多价纳米药物的时代，其工程跨度-激发的治疗剂和强效疫苗的应用尚未受到重视。这篇文章简要介绍了SrtA酶及其相关的跨肽反应活性，并描述了有趣的通过sortase介导的蛋白质工程和化学方法以实现多价治疗和抗原反应的途径。该回顾还突出了在定向递送系统、多价治疗、细胞疗法和疫苗工程方面的先进应用。这些创新展示了sortase介导技术在促进针对难治性疾病和流感等大流行病的简单即插即用纳米医学技术发展方面的潜力。版权所有©2023 Elsevier Inc.。保留所有权利。

The engineering of potent prophylactic and therapeutic complexes has always required careful protein modification techniques with seamless capabilities. In this light, methods that favor unobstructed multivalent targeting and correct antigen presentations remain essential and very demanding. Sortase A (SrtA) transpeptidation has exhibited these attributes in various settings over the years. However, its applications for engineering avidity-inspired therapeutics and potent vaccines have yet to be significantly noticed, especially in this era where active targeting and multivalent nanomedications are in great demand. This review briefly presents the SrtA enzyme and its associated transpeptidation activity and describes interesting sortase-mediated protein engineering and chemistry approaches for achieving multivalent therapeutic and antigenic responses. The review further highlights advanced applications in targeted delivery systems, multivalent therapeutics, adoptive cellular therapy, and vaccine engineering. These innovations show the potential of sortase-mediated techniques in facilitating the development of simple plug-and-play nanomedicine technologies against recalcitrant diseases and pandemics such as cancer and viral infections.Copyright © 2023 Elsevier Inc. All rights reserved.