该研究探讨了利用绿色原料Opuntia cactus果实提取物合成的硫化镉纳米颗粒(CdS NPs)的多功能性质，包括其杀虫、抗菌和抗癌活性。不同浓度(0.005-0.04%)的CdS NPs被喂食给二化螟幼虫，观察它们在不同的生物、生化和氧化应激标志物上的摄入效果。处理后的幼虫中，超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽S-转移酶(GST)和MDA水平这类生化参数的显著剂量依赖性变化得到了研究。在最高浓度(0.04%)，相比对照组，观察到显著的幼虫死亡率(46.66%)、畸形率(蛹和成虫)(27.78%)、成虫出现抑制率(43.87%)，以及繁殖力(25.28%)和生育力(22.74%)的降低。CdS NPs被研究用于对抗铜绿假单胞菌和金黄色葡萄球菌等菌株。在体内的抗癌活性试验中，对胰腺癌细胞系的存活率下降达到了18%和12%，分别在与CdS NPs孵育24和48小时以后，证实了其强有效的抗癌特性。对红细胞(RBC)毒性的缺乏支持了合成的CdS NPs具有生物相容性的结论。研究发现，绿色合成的CdS NPs可以作为一种有前途的杀虫、抗菌和抗癌剂。版权所有©2022 Elsevier Inc.。

The current study investigated the multifunctional properties of Cadmium Sulphide Nanoparticles synthesized using a green synthesis method (CdS NPs) using a green feedstock, Nopal Cactus fruit extract. The biological activities of the CdS NPs were thoroughly investigated, including their insecticidal, antibacterial, and anticancer activities. The different concentrations (0.005-0.04%) of CdS NPs were fed to the larvae of Spodoptera litura, and their ingestion effects were observed on the different biological, biochemical, and oxidative stress markers. There are significant dose-dependent changes in the biochemical parameters like superoxide dismutase (SOD), Catalase (CAT), Glutathione-S-transferase (GST), and MDA level as a marker of lipid peroxidation in the treated larvae were studied. In the highest concentration (0.04%), significant larval mortality (46.66%), malformation (pupae and adult) (27.78%), inhibition of adult emergence (43.87%), as well as reduced fecundity (25.28%), and fertility (22.74%) as compared to control was observed. CdS NPs have been investigated for antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus bacterial strains. In vitro anticancer activities were carried out to decrease the viability of the Pancreatic cancer cell line. The cells showed 18% and 12% viability at a 200 μg/ml concentration when incubated with CdS NPs for 24 and 48 h, respectively, confirming its potent anticancer property. The lack of cytotoxicity against the (RBC) endorses the biocompatible nature of synthesized CdS NPs. It was observed that green synthesized CdS NPs could be used as a promising insecticidal, antibacterial, and anticancer agent.Copyright © 2022 Elsevier Inc. All rights reserved.