哺乳动物中，右非开放阅读框激酶（RIOKs）最初被报道参与癌细胞增殖、凋亡、迁移和侵袭，近期又被发现与宿主免疫反应有关。对于鱼类中RIOKs的同源物鲜有研究。本研究在黄鱼（Pelteobagrus fulvidraco）中克隆了RIOK家族的三个同源基因，分别命名为Pfriok1、Pfriok2和Pfriok3。Pfriok1、Pfriok2和Pfriok3在黄鱼组织中表达水平相对较高，并且它们的mRNA水平在病毒感染下没有变化。PfRIOK1、PfRIOK2和PfRIOK3的个体过度表达削弱了鱼类干扰素（IFN）的反应，从而促进了鱼类细胞中病毒的复制。从机理上讲，黄鱼RIOK蛋白质通过减弱胞质中TBK1蛋白质水平，下调了鱼类IFN的反应。我们的研究结果表明，在下调鱼类IFN抗病毒反应方面，黄鱼RIOK1、RIOK2和RIOK3发挥了作用。版权所有 © 2023 Elsevier Ltd.

In mammals, right open reading frame kinases (RIOKs) are initially reported to participate in cancer cell proliferation, apoptosis, migration and invasion, and recently they have been related to host immune response. Little is known about the homologs of RIOKs in fish. In the current study, we cloned three homologous genes of RIOK family in yellow catfish (Pelteobagrus fulvidraco), termed Pfriok1, Pfriok2 and Pfriok3. Pfriok1, Pfriok2 and Pfriok3 were constitutively expressed at relatively high levels in yellow catfish tissues, and their mRNA levels were not changed under viral infection. Individual overexpression of PfRIOK1, PfRIOK2 and PfRIOK3 attenuated fish interferon (IFN) response, thereby promoting viral replication in fish cells. Mechanistically, yellow catfish RIOK proteins downregulated fish IFN response through attenuating TBK1 protein levels in cytoplasm. Our findings suggest that yellow catfish RIOK1, RIOK2 and RIOK3 are involved in downregulating fish IFN antiviral response.Copyright © 2023 Elsevier Ltd. All rights reserved.