肿瘤微环境（TME）由许多不同的细胞和非细胞组成部分组成，共同推动肿瘤的生长、侵袭、转移和对治疗的反应。越来越多的人意识到TME在癌症生物学中的重要性，已经将癌症研究从以癌症为中心的模式转变为整体考虑TME。最近空间分析方法技术的进步提供了系统全面地查看和阐明TME成分的物理位置。本篇综述文章，我们提供了主要的空间分析技术概述，描绘了这些数据中可提取的信息类型，并阐述了它们在癌症研究中的应用、发现和挑战。最后，我们提供了一个未来的展望，如何将空间分析技术整合到癌症研究中，从而改善患者的诊断、预后、治疗分层和开发新的治疗方法。版权所有©2023 Elsevier Inc.。

The tumor microenvironment (TME) is composed of many different cellular and acellular components that together drive tumor growth, invasion, metastasis, and response to therapies. Increasing realization of the significance of the TME in cancer biology has shifted cancer research from a cancer-centric model to one that considers the TME as a whole. Recent technological advancements in spatial profiling methodologies provide a systematic view and illuminate the physical localization of the components of the TME. In this review, we provide an overview of major spatial profiling technologies. We present the types of information that can be extracted from these data and describe their applications, findings and challenges in cancer research. Finally, we provide a future perspective of how spatial profiling could be integrated into cancer research to improve patient diagnosis, prognosis, stratification to treatment and development of novel therapeutics.Copyright © 2023 Elsevier Inc. All rights reserved.