目前的研究表明，在感染水气单胞菌（Aeromonas hydrophila）的草鱼（Ctenopharyngodon Idella）中，miR-217调节炎症。草鱼的细菌感染会引起高水平的败血症，并伴随全身性炎症反应。结果导致过度炎症状态的发展，进而引起败血性休克和致死性疾病。根据目前的数据，在成功进行基因表达分析实验或荧光素酶实验以及草鱼CIK细胞中miR-217表达之后，已确认TBK1是miR-217的靶基因。此外，TargetscanFish6.2预测了TBK1是miR-217的靶基因。在CIK细胞中，进行实时荧光定量PCR来测量免疫相关基因和miR-217在感染A. hydrophila后在草鱼中的调节水平。在草鱼CIK细胞中，聚（I：C）刺激下，TBK1 mRNA表达上调。免疫相关基因的转录分析表明，在成功转染CIK细胞后，肿瘤坏死因子-α（TNF-α），干扰素ifn，白细胞介素6（IL-6），白细胞介素8 （il-8）和白细胞介素12（IL-12）的表达水平发生变化，暗示miRNA在调节草鱼的免疫反应。这些结果为A. Hydrophila感染病理机制和宿主防御系统的进一步研究提供了理论基础和贡献。版权所有©2022 Elsevier Ltd.已发表。

The current study demonstrated that miR-217 modulate inflammation in grass carp (Ctenopharyngodon Idella) infected with Aeromonas hydrophila. Bacterial infection in grass carp causes high level of septicemia which arises with systemic inflammatory responses. As a result leading to the development of hyper inflammatory state which causes septic shocks and lethality. Base on the current data, TBK1 was confirmed to be the target gene of miR-217 after a successful gene expression profiling experiment or luciferase experiment and miR-217 expression in CIK cells. Furthermore, TargetscanFish6.2 predicted TBK1 as the target gene of miR-217. Quantitative real-time PCR was performed to measure miR-217 expression levels for six immune related genes and miR-217 regulation in grass carp after A. hydrophila infection in CIK cells. In grass carp CIK cells, the expression of TBK1 mRNA was up-regulated under poly (I: C) stimulation. The transcriptional analysis of the immune related genes demonstrated that the expression levels of tumor necrosis factor-α (TNF-α), interferon ifn, interleukin 6 (il-6), interleukin 8 (il-8) and interleukin 12 (il-12) were altered after a successful transfection in to the CIK cells, proposing that miRNA regulates immune responses in grass carp. These results provided a theoretical basis and contribute to further studies on the pathogenesis and host defensive system during A. hydrophila infection.Copyright © 2022. Published by Elsevier Ltd.