在乳腺癌治疗中，铱柔比星（EPI）是一种高度细胞毒性的蒽环素类药物。色氨酸衍生的3-吲哚丙酸（3-IPA）可以减少氧化损伤。我们研究了3-IPA在大鼠下丘脑-卵巢-子宫轴中作为减轻EPI诱导的细胞毒性的潜力。雌性大鼠分为四组：对照组、EPI组（2.5毫克/千克）、单独应用3-IPA组（40毫克/千克）、联合处理EPI和3-IPA的两个组（EPI+3-IPA1组2.5毫克/千克+20毫克/千克、EPI+3-IPA2组2.5毫克/千克+40毫克/千克），持续28天。然后检测了生殖激素、氧化和炎症应激标志物以及组织组织学。3-IPA可以预防EPI诱导的卵泡刺激素、雌二醇、孕激素和催乳素水平的下降。3-IPA联合处理可以部分抵消EPI介导的抗氧化酶、还原型谷胱甘肽和总巯基组的降低。EPI处理引起的氧化和炎症应激标志物的增加也可以通过3-IPA联合处理减轻。此外，3-IPA也可以减轻组织的组织学损伤。总之，通过抗氧化和抗炎机制，并稳定血清激素动态，3-IPA可以减轻EPI处理所致的生化标志物和组织损伤。Copyright © 2023 Elsevier B.V. All rights reserved.

The "anthracycline, Epirubicin (EPI)," in managing breast cancer, is highly cytotoxic. Tryptophan-derived 3-indolepropionic acid (3-IPA) decreases oxidative damage, and its prospect of alleviating EPI-induced cytotoxicity was examined in rats' hypothalamus-ovary-uterus axis. Female rats: Control, EPI (2.5 mg/kg), 3-IPA alone (40 mg/kg), EPI+3-IPA (2.5 mg/kg + 20 mg/kg), EPI + 3-IPA2 (2.5 mg/kg + 40 mg/kg) were treated for 28 days. Subsequently, reproductive hormones, oxidative and inflammatory stress biomarkers, and tissue histology were examined. 3-IPA prevented EPI-induced decreases in the follicle-stimulating hormone, estradiol, progesterone and prolactin levels. EPI-mediated reduction in antioxidant enzymes, reduced glutathione and total sulfhydryl groups were partially counteracted by 3-IPA co-treatment. Increased oxidative and inflammatory stress biomarkers caused by treatment with EPI alone were lessened by 3-IPA co-treatment. Also, 3-IPA reduced histological damage in the examined tissues. Conclusively, 3-IPA ameliorated biochemical markers and tissue injury caused by EPI treatment alone via an antioxidative and anti-inflammatory mechanism while stabilising serum hormone dynamics.Copyright © 2023 Elsevier B.V. All rights reserved.