细胞外囊泡（EVs）被期望作为有趣的药物输送载体，因为它们可以提供药物输送的独特和新的特性。它们的天然来源、蛋白质和核酸组成，以及内在的多效性治疗作用可能在药物输送领域开启新的可能性。在这里，我们的目标是回顾用于制备混合EVs的方法，混合EVs是一种最近出现的基于EVs和合成载体制作的向量类型，可以利用它们各自的特性。混合EV/合成物可以通过孵育、静电相互作用、聚乙二醇（PEG）介导的融合、共挤出、冷冻-融化或简单的EV表面修饰来获得，从而导致不同类型的物体。我们还选择回顾这些向量的特性，并与其他药物输送向量进行了比较。需要注意的是，只有有限的研究报告允许跨文章比较的负载指标。基于这个批判性的分析，我们尝试从这些相对难以比较的研究中提取出精髓，并将它们整合到更广泛的药物输送和药物开发的机会中，特别关注肿瘤学。版权所有 ©2023 Elsevier B.V. 发布。

Extracellular vesicles (EVs) are expected to serve as interesting drug delivery vectors as they may offer unique and new properties for drug delivery. Their natural origin, protein and nucleic acid composition, and intrinsic pleiotropic therapeutic effects could enable new possibilities in the field of drug delivery. Here, we aimed to review the methods used to produce Hybrid EVs, a recently emerged type of EV-based vector made from both EVs and synthetic vectors to exploit their respective properties. Hybrid EV/synthetic objects can be obtained by incubation, electrostatic interactions, polyethylene glycol (PEG)-mediated fusion, co-extrusion, freeze-thawing, or simple EV surface modification, leading to different types of objects. We also opted to review the properties of these vectors, and specifically compared them with those of other drug delivery vectors. It has to be noticed that only a limited number of study report loading metrics that allow cross article comparison. Based on this critical analysis, we attempted to draw the pith and marrow from these relatively difficult-to-compare studies and integrate them into the more general context of opportunities in drug delivery and drug development, with a particular focus on oncology.Copyright © 2023. Published by Elsevier B.V.