从健康人外周血单个核细胞中体外扩增Wilms瘤蛋白1表位特异性初级T细胞。
In vitro expansion of Wilms' tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells.
发表日期：2023 Jan 30
作者：
Sanne van der Heijden, Donovan Flumens, Maarten Versteven, Stefanie Peeters, Hans De Reu, Diana Campillo-Davo, Yannick Willemen, Benson Ogunjimi, Viggo Van Tendeloo, Zwi N Berneman, Sébastien Anguille, Evelien Smits, Eva Lion
来源：
Experimental Hematology & Oncology
摘要：
威尔姆斯肿瘤蛋白1（WT1）是一种在各种癌症中过度表达的肿瘤相关抗原。作为一种自身抗原，负选择会减少WT1特异性T细胞受体（TCR）的数量。我们提供了一种使用健康人外周血单个核细胞制备WT137-45特异性TCR的方案。我们描述了通过两个连续的体外刺激，使用同源WT137-45脉冲树突状细胞和外周血淋巴细胞扩增WT1特异性T细胞克隆。然后我们详细介绍了使用人白细胞抗原/WT137-45四聚体进行检测的方法。 版权所有© 2023年作者。由Elsevier Inc.出版并保留所有权利。
Wilms' tumor protein 1 (WT1) is a tumor-associated antigen overexpressed in various cancers. As a self-antigen, negative selection reduces the number of WT1-specific T cell receptors (TCRs). Here, we provide a protocol to generate WT137-45-specific TCRs using healthy human peripheral blood mononuclear cells. We describe the expansion of WT1-specific T cell clones by two consecutive in vitro stimulations with autologous WT137-45-pulsed dendritic cells and peripheral blood lymphocytes. We then detail the detection with human leukocyte antigen/WT137-45 tetramers.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.