石墨炔氧化物量子点（GDYO QDs）是石墨炔（GDY）的衍生物，具有出色的电导和发光性能，可用作新的电化学发光（ECL）发射体。本研究基于AuNPs / GDY，GDYO QD和寡核苷酸信号放大策略构建一个miRNA-21超灵敏检测的ECL生物传感器，该策略集成了DNA Walker和杂交链反应（HCR）放大技术。作为电极基底材料，AuNPs / GDY不仅能与合适的CP缘分牢固，还可以增强接口的电导性。当miRNA-21存在时，DNA Walker过程被启动，信号探针被引入到电极表面，产生丰富的双链H1 / H2;然后，H3 / H4通过HCR与H1 / H2进行互补碱基配对。随着miRNA-21的增加，3D DNA纳米器被积极地操纵，结果会产生逐渐增加的ECL信号。该ECL生物传感器在0.1 fM到1 nM的线性范围内检测miRNA-21表现出卓越的性能。该研究为癌症生物标志物的临床分析提供了新的敏感思路。

Graphdiyne oxide quantum dots (GDYO QDs), as derivatives of graphdiyne (GDY), have excellent electroconductibility and luminous properties and can be applied as a new ECL emitter. Herein, an electrochemiluminescence (ECL) biosensor for miRNA-21 ultrasensitive determination is constructed based on AuNPs/GDY, GDYO QD and oligonucleotide signal amplification strategy that integrates DNA walker and hybridization chain reaction (HCR) amplification. As electrode substrate material, AuNPs/GDY can not only bond with the aptamer CP but can also enhance the conductivity of the interface. When miRNA-21 exists, the DNA walker process is initiated, and the signaling probes are introduced on the electrode surface, producing abundant double-stranded H1/H2; then, H3/H4 undergoes complementary base pairing with H1/H2 through HCR. With the increase in miRNA-21, the 3D DNA nanomachine is actively manipulated, resulting in a gradual increase in ECL signal. This ECL biosensor demonstrates outstanding performance in the determination of miRNA-21 in the linear range from 0.1 fM to 1 nM. This study offers a new sensitive idea for the clinical analysis of cancer biomarkers.