趋化因子受体系统在白细胞交通、炎症、免疫细胞分化、癌症和其他生物过程中发挥重要作用。本研究分析了大嘴黑鲈（Micropterus salmoides）的12种CXC趋化因子配体（CXCL8a.1、CXCL8a.2、CXCL8b.1、CXCL8b.2、CXCL12a、CXCL12b、CXCL13.1、CXCL13.2、CXCL14、CXCL18a、CXCL18b和CXCL19）和8种CXC趋化因子受体（CXCR1、CXCR2、CXCR3.1、CXCR3.2、CXCR3.3、CXCR4a、CXCR4b和CXCR5）的序列特征、结构和表达模式。大嘴黑鲈的所有CXCL和CXCR与其鲇鱼同源物有很高的序列同源性，并具有CXCL和CXCR家族的保守基序和结构。实时荧光定量PCR表明，在所有检测到的组织中，这些CXCL和CXCR均具有广泛的表达，且在免疫相关组织（脾、头部肾和鳃）中表达水平较高。在脾脏经过脂多糖（LPS）和聚肌核苷酸-多聚胞苷酸（polyI：C）刺激后，大多数这些CXCL和CXCR在脾脏中显着上调。此外，通过蛋白质相互作用网络分析，分析了这些CXCL和CXCR的潜在相互作用分子。据我们所知，这是首次对大嘴黑鲈的CXCL和CXCR进行详细分析。我们的研究结果为研究趋化因子受体系统在大嘴黑鲈中的功能和机制提供了有价值的基础。Copyright © 2023. Elsevier Ltd.发表

The chemokine-receptor system plays important roles in the leukocyte trafficking, inflammation, immune cell differentiation, cancer and other biological processes. In the present study, the sequence features, structures and expression patterns of twelve CXC chemokine ligands (CXCL8a.1, CXCL8a.2, CXCL8b.1, CXCL8b.2, CXCL12a, CXCL12b, CXCL13.1, CXCL13.2, CXCL14, CXCL18a, CXCL18b and CXCL19) and eight CXC chemokine receptors (CXCR1, CXCR2, CXCR3.1, CXCR3.2, CXCR3.3, CXCR4a, CXCR4b and CXCR5) of largemouth bass (Micropterus salmoides) were analyzed. All the CXCLs and CXCRs of largemouth bass shared high sequence identities with their teleost counterparts and possessed conserved motifs and structures of CXCLs and CXCRs family. Realtime qPCR revealed that these CXCLs and CXCRs were ubiquitously expressed in all examined tissues, with high expression levels in the immune-related tissues (spleen, head kidney, and gill). Following lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (polyI:C) stimulations, most of these CXCLs and CXCRs were significantly up-regulated in spleen. In addition, the potential interacted molecules of these CXCLs and CXCRs were analyzed by protein-protein interaction network analysis. To the best of our knowledge, this is the first study that in detail analyzes the CXCLs and CXCRs of largemouth bass. Our results provide valuable basis for study the function and mechanism of chemokine-receptor system in largemouth bass.Copyright © 2023. Published by Elsevier Ltd.