沸石咪唑框架67（ZIF-67）已被赋予天然生物材料和DNA来开发一种有前途的策略和适合且安全的联合输送平台，用于Doxorubicin和Sorafenib（DOX-SOR）的联合输送。采用FT-IR、XRD、FESEM和TEM对改性金属有机骨架进行表征。采用银杏叶提取物和大肠杆菌DNA作为绿色点缀，将DOX和SOR附着在MOFs的孔隙中。TEM和FESEM图像证明成功合成了用于生物医学应用的绿色MOFs，其呈现正方体结构。由于纳米载体和药物之间的相互作用，DOX和SOR的药物负载分别达到了59.7%和60.2%。HEK293、HT29和MCF-7细胞在低浓度和高浓度（0.1和50 μg/mL）DNA和银杏叶提取物修饰下都显示出良好的存活，表明它们可以在生物医学应用中使用。MTT实验证明纳米载体与正常细胞高度相容，对HT29和MCF-7细胞具有抗癌特性。由于银杏叶提取物和DNA的修饰，DOX-SOR释放时间延长，具有pH敏感性（在pH 4.5和5.5下释放最强）。利用共聚焦激光显微镜研究了药物的内部化和输送，证明药物被有效地内部化。细胞图像显示纳米粒子在MCF-7细胞中内部化，证明其作为药物输送系统的功效。版权所有 © 2023 Elsevier Inc.

Zeolitic imidazolate framework-67 (ZIF-67) has been decorated with natural biomaterials and DNA to develop a promising strategy and suitable and safe co-delivery platform for doxorubicin and sorafenib (DOX-SOR). FT-IR, XRD, FESEM, and TEM were used to characterize the modified MOFs. Combined Ginkgo biloba leaf extract and E. coli DNA were used as green decorations, and DOX and SOR were attached to the porosity of the MOFs. TEM and FESEM images demonstrated that the green MOFs were successfully synthesized for biomedical applications and showed their cubic structure. As a result of the nanocarrier-drug interactions, 59.7% and 60.2% of the drug payload were achieved with DOX and SOR, respectively. HEK-293, HT-29, and MCF-7 cells displayed excellent viability by decoration with DNA and Ginkgo biloba leaf extract at low and high concentrations (0.1 and 50 μg/mL), suggesting they could be used in biomedical applications. MTT assays demonstrated that the nanocarriers are highly biocompatible with normal cells and possess anticancer properties when applied to HT-29 and MCF-7 cells. As a result of Ginkgo biloba leaf extract and DNA modification, DOX-SOR release was prolonged and pH-sensitive (highest release at pHs 4.5 and 5.5). The internalization and delivery of the drug were also studied using a confocal scanning laser microscope, demonstrating that the drug was effectively internalized. Cell images showed NPs internalizing in MCF-7 cells, proving their efficacy as drug delivery systems.Copyright © 2023. Published by Elsevier Inc.