由于与其他污染物一起存在的木质素使得制浆造纸厂排放的废水更加复杂。本研究探讨了从制浆造纸厂污染现场（PPMECSs）中分离出的生物膜形成菌对木质素的降解作用。通过16S rRNA基因测序鉴定了分离的具有生物膜形成和木质素降解能力的细菌种类为枯草芽孢杆菌、癌变肠杆菌和地衣芽孢杆菌。薄层液相色谱分析显示，这些细菌共生体产生了酰基-同半胱氨酸内酯（AHL）作为群体感应分子和外生聚合物物质（EPS），后者可在不利条件下保护细菌共生体。相比单一菌株，这个菌群共生体在pH 7.0和37 °C下以含kraft木质素和葡萄糖的最小盐培养基中培养8天后，能将木质素（900 ppm）降解73％。降解产物为酰胺，醇和酸。构建的菌群共生体处理废水后，可显著降低废水中的有机污染物，这证实了木质素的活性生物转化和生物降解。显微镜检查还表明有木质素诱导的生物膜形成。因此，基于群体感应构建的生物膜形成菌群提供了一种可持续和有效处理含木质素复杂污染物的解决方案。版权所有 © 2023 Elsevier Inc. 发布。

Due to presence of lignin along with other pollutants makes effluent more complex which is discharged from Pulp and paper mills. The present study investigates the use of biofilm-forming bacteria isolated from pulp paper mill effluent contaminated sites (PPMECSs) for lignin degradation. Isolated biofilm-forming and lignin-degrading bacteria were identified as Bacillus subtilis, Enterobacter cancerogenus, and Bacillus licheniformis by 16S rRNA gene sequencing. Thin liquid chromatography (TLC) analysis showed that the consortium of bacteria produced acyl-homoserine lactone (AHL) as quorum sensing molecules and extracellular polymeric substances (EPS) that protect the bacterial consortium under unfavorable conditions. The potential consortium was able to reduce lignin (900 ppm) by 73% after 8 days of incubation in a minimal salt medium containing kraft lignin and glucose at pH 7.0 and 37 °C as compared to individual strains. The degradation by-products were identified as amides, alcohols, and acids. The major organic pollutants in the effluent were reduced after treatment of the constructed consortium, thus confirming active biotransformation and biodegradation of the lignin. Microscopic examination also indicated the presence of lignin induced biofilm formation. Hence, the constructed biofilm-forming bacterial consortia based on quorum sensing offered a sustainable and effective solution to treat lignin-containing complex pollutants.Copyright © 2023. Published by Elsevier Inc.