磺酸化多糖是有效的免疫刺激剂，可以通过激活几种细胞内信号通路实现。从海洋大型藻类紫菜蝴蝶贝壳藻中纯化出了一种具有免疫调节功效的磺酸化(1 → 3)/(1 → 4)-连接的半乳糖褐藻酸TCP-3。通过提高骨髓细胞数量（每股骨/ml x 106 10-13个细胞）、α-酯酶活性（4000个BMC中1200-1700个阳性细胞）、干扰素γ（1.31-1.49 pg/ml）、白细胞计数（>8000个/cu mm）评估了TCP-3(100-400 mg/kg BW)在环磷酰胺诱导的免疫抑制动物身上的免疫增强潜力。TCP-3的投与不仅可以增强体外和体内条件下淋巴细胞的增殖，还可以调节促炎细胞因子(白细胞介素-6/1β/12、肿瘤坏死因子-α、转化生长因子-β)和诱导型NO合酶。与感染组(>5 × 107数量)相比，TCP-3(<2 × 107数量)投与第三波新冠病毒Delta菌株感染vero细胞，有望显著减少病毒复制。 版权所有 ©2023 Elsevier B.V. 发布。

Sulfated polysaccharides are effective immunostimulating agents by activating several intracellular signaling pathways. A sulfated (1 → 3)/(1 → 4)-linked galactofucan TCP-3 with promising immunomodulatory effects was purified from a marine macroalga Turbinaria conoides. The immune-enhancing potential of TCP-3 (100-400 mg/kg BW) was evaluated on cyclophosphamide-induced immunosuppressed animals by increasing bone marrow cellularity (10-13 cells/femur/mL x 106), α-esterase activity (1200-1700 number of positive cells/4000 BMC), interferon-γ (1.31-1.49 pg/mL), interleukin-2 (3.49-3.99 pg/mL) secretion, and WBC count (> 8000 cells/cu mm). The proliferation of lymphocytes for in vitro and in vivo conditions was enhanced by administering TCP-3 besides regulating the secretion of pro-inflammatory cytokines (interleukin-6/1β/12, tumor necrosis factor-α, transforming growth factor-β), and an inducible isoform of nitric oxide synthase. A promising reduction of viral copy formation was observed by administering TCP-3 (< 2 × 107 number) on SARS CoV-2 (delta variant) induced Vero cells in comparison with the infected group (> 5 × 107 number).Copyright © 2023. Published by Elsevier B.V.