人工甜味剂被用作无热量的糖替代品在许多食品产品中，它们的消费量在过去的几年内大幅增长。虽然一般被认为是安全的，但一些关于某些甜味剂的长期安全性的担忧已经被提出。在这项研究中，我们展示了在高剂量下摄入蔗糖精导致小鼠免疫调节性的影响，通过限制T细胞增殖和T细胞分化。机制上，蔗糖精影响T细胞膜有序性，伴随着T细胞受体信号和细胞内钙离子动员效率的降低。给予蔗糖精的小鼠在皮下癌模型和细菌感染模型中显示出降低的CD8+ T细胞特定抗原反应，以及在T细胞介导的自身免疫模型中降低的T细胞功能。总的来说，这些发现表明高摄入蔗糖精可以抑制T细胞介导的反应，在治疗缓解T细胞依赖的自身免疫性疾病方面可能会有作用。©2023作者。

Artificial sweeteners are used as calorie-free sugar substitutes in many food products and their consumption has increased substantially over the past years1. Although generally regarded as safe, some concerns have been raised about the long-term safety of the consumption of certain sweeteners2-5. In this study, we show that the intake of high doses of sucralose in mice results in immunomodulatory effects by limiting T cell proliferation and T cell differentiation. Mechanistically, sucralose affects the membrane order of T cells, accompanied by a reduced efficiency of T cell receptor signalling and intracellular calcium mobilization. Mice given sucralose show decreased CD8+ T cell antigen-specific responses in subcutaneous cancer models and bacterial infection models, and reduced T cell function in models of T cell-mediated autoimmunity. Overall, these findings suggest that a high intake of sucralose can dampen T cell-mediated responses, an effect that could be used in therapy to mitigate T cell-dependent autoimmune disorders.© 2023. The Author(s).