光动力疗法(PDT)用于治疗皮肤癌，可以通过直接和间接的方式诱导细胞死亡，包括凋亡、炎症和某些免疫机制，穿透深度可能是潜在的修饰因素。为了研究基底细胞癌(BCC)和皮内鳞状细胞癌(Bowen病)中凋亡通路的影响，对16名患有浅表性或结节性BCC和Bowen病的患者进行了局部给予δ-氨基酮戊酸-PDT治疗。在基线和PDT 24小时后采取活检组织，通过免疫组化检查凋亡标记物的表达，如半胱氨酸天冬酶3参与内在性凋亡途径、生长抑素B是一种半胱氨酸无关的凋亡介质以及促凋亡标记物BAX和BAK。结果：TUNEL染色显示，PDT 24小时后出现明显凋亡细胞染色(p<0.01在BCC和Bowen病的病变区域中均如此)。PDT 24小时后，半胱氨酸天冬酶3(p<0.01在BCC和p<0.05在Bowen's中)和生长抑素B(p<0.01在BCC和p<0.01在Bowen's中)显著升高。在Bowen's 病变处24小时PDT后，BAX表达显著提高，而Bak在付出PDT后的BCC和Bowen's病变处的基线和24小时均有上调。通过应用局部PDT，可通过公共和选择性的凋亡途径介导BCC和Bowen's疾病中的细胞凋亡，其中涉及生长抑素B的促凋亡因素在BCC和Bowen's病变中均增加，而在Bowen's疾病中BAX的增加表现出明显上调。本文受版权保护，所有权利均归原作者所有。

Photodynamic therapy (PDT) is used to treat cutaneous cancers. It may induce cell death through direct and indirect means, including apoptosis, inflammation, and certain immune mechanisms, with the depth of penetration as a potential modifying factor.To examine the pathways of apoptosis in the intralesional PDT of basal cell carcinoma (BCC) and intraepidermal squamous cell carcinoma (Bowen's disease).Sixteen patients with superficial or nodular BCC and Bowen's disease were treated with intralesional aminolevulinic acid-PDT. Biopsies were taken at baseline and 24 h post-PDT and sections were examined by immunohistochemistry for expression of markers of apoptosis. such as caspase 3, involved in the intrinsic apoptotic pathway, granzyme B, a caspase-independent apoptotic mediator, and the proapoptotic markers BAX and BAK RESULTS: Apoptotic cells stained with TUNEL showed statistically significant staining at 24 h post PDT (p<0,01 in both BCC and Bowen's lesions). Caspase 3 (p<0.01 in BCC and p<0.05 in Bowen's) and granzyme B (p<0.01 in BCC and p<0.01 in Bowen's) were significantly increased at 24 h post-PDT. BAX expression was apparently raised compared to baseline in Bowen's lesions at 24 h post-PDT, whereas Bak was upregulated both in BCC and Bowen's disease at baseline and at 24 h post-PDT.Intralesional PDT induces apoptosis in BCC and Bowen's disease via common and alternative apoptotic pathways involving granzyme B. Proapoptotic factors Bak in both BCC and Bowen and Bax in Bowen's disease appear to increase by intralesional PDT at 24 hours.This article is protected by copyright. All rights reserved.