为探究紫花地丁（PV）与柴胡（RB）联合对乳头状甲状腺癌细胞凋亡的影响，我们将研究分为四组：对照组、PV组、RB组、PV联合RB组。通过CCK-8试验评估不同处理组的细胞活力，采用愈合创伤和Transwell实验评估细胞迁移和侵袭，采用流式细胞术检测细胞凋亡率和细胞周期阻滞。通过Western blot实验检测Bcl-2、Bax、Caspase-3、CyclinA1、CyclinB1和CDK1等蛋白质的表达。结果表明，与对照组相比，PV联合RB组能够显著改变细胞形态，抑制细胞迁移和侵袭，减少G0/G1期细胞数，增加G2/M期细胞数，促进细胞凋亡。此外，PV联合RB处理也明显增加了Bax/Bcl2和Caspase-3蛋白的表达，减少了Cyclin A1、Cyclin B1和CDK1蛋白的表达。总的来说，我们的结果表明，PV联合RB可激活Bax/Bcl-2和Caspase-3信号通路诱导乳头状甲状腺癌细胞凋亡，这也为治疗甲状腺癌提供了新途径。

To explore the effect of Prunella vulgaris (PV) combined with Radix bupleuri (RB) on apoptosis of papillary thyroid carcinoma cells. Our study was divided into four groups: the control group, the PV group, the RB group, and the PV combined with the RB group. The viability of cells from different treatment groups was assessed by the CCK-8 assay. Cell migration and invasion were assessed by healing wounding and the transwell assay, respectively. Cell apoptosis rate and cell cycle arrest were detected by a flow cytometry assay. The protein expression of Bcl-2, Bax, Caspase-3, CyclinA1, CyclinB1, and CDK1 was detected using a western blot assay. Our results indicated that, compared with the control group, PV combined with RB group could significantly alter the cell morphology, inhibit cell migration and invasion, decrease the number of cells in the G0/G1 phase and increase the number of cells in the G2/M phase, and promote the cell apoptosis. Moreover, PV combined with RB treatment also obviously increased the expression of Bax/Bcl2 and caspase-3 proteins and decreased the expression of Cyclin A1, Cyclin B1, and CDK1 proteins. Overall, our results indicated that PV combined with RB could activate the Bax/Bcl-2 and Caspase-3 signal pathways to induce cell apoptosis in papillary thyroid carcinoma cells; this also provides a new way to treat thyroid cancer.