脑器官样体是研究人类皮层发育、进化、生理学、功能和疾病机制的最佳实验系统。在此，我们描述了将人类多能干细胞（hPSCs）分化为脑器官样体的简单方案。我们描述了hPSC维持、胚胎体神经诱导以及脑器官样体花纹形成的步骤。我们还详细介绍了对hPSC源脑器官样体中的每个神经管状区域进行表型分析的过程。有关使用和执行此方案的完整细节，请参阅唐等（2021）。1.版权所有 © 2023作者。由Elsevier Inc.出版。保留所有权利。

Cerebral organoids represent an optimal experimental system for studying human cortical development, evolution, physiology, function, and disease mechanisms. Here, we describe a simple protocol for the differentiation of human pluripotent stem cells (hPSCs) into cerebral organoids. We describe steps for hPSC maintenance, neural induction of embryoid bodies, and patterning of cerebral organoids. We also detail a process for the phenotypic assay of each neural-tube-like area in hPSC-derived cerebral organoids. For complete details on the use and execution of this protocol, please refer to Tang et al. (2021).1.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.