现成的多基因型体外诊断对于预防和治疗宫颈癌非常重要。在此，我们提出了一种基于纸膜的交互式双读数差分侧向流生物传感器，可同时检测人乳头瘤病毒（HPV）的两种高危型。CuCo2S4/ZnIn2S4异质结构被制造在纸膜复合芯片上，具有高热稳定性，并通过轻微退火处理引入表面硫缺陷，赋予了无与伦比的光促活性，这种结构可作为初步的能量收集器和转换器。在差分通道的辅助下，序列性溶液转移逐步激活细胞环化DNA的自组装和CRISPR-Cas12a的裂解活性。因此，带有光热/光电特性的聚多巴胺包覆的金纳米颗粒的输入和释放被实现。制造的生物传感器不仅实现了智能热响应而无需大型仪器，而且激活了动态界面电荷分离和传递动力学，进一步传输光电信号，从而产生了理想的交互双信号，其最低检测限为0.21 pM（HPV-18）和42.92 pM（HPV-16）。由于差分侧向流纸膜复合芯片和交互式双信号放大策略的精密设计，实现了对两种HPV基因型的敏感检测，为家庭医疗智能诊断提供了一个有前途的候选者。版权所有©2023 Elsevier B.V.保留所有权利。

Ready-to-use in vitro diagnosis of multiple genotypes is vital for the prevention and treatment of cervical cancer. Herein, a paper-film-based interactive dual readout differential lateral flow biosensor is proposed to simultaneously assay two high-risk types of human papillomavirus (HPV) within the body-fluid. The CuCo2S4/ZnIn2S4 heterostructure is fabricated on the paper-film compound chip with high thermostability, and surface sulfur vacancy is introduced by mild annealing treatment to endow unexceptionable photoexcitation activity, such structure can be served as an initial energy harvester and converter. With the assistance of differential channels, the dual-target-propelled self-assembly of annular DNA and the cleavage activity of CRISPR-Cas12a are stepwise activated by sequential solution transfer. Accordingly, the input and release of polydopamine-coated gold nanoparticles with photothermal/photoelectric characteristic were implemented. The fabricated biosensor not only realized intelligent thermal-response without large instruments, but also actuated dynamic interfacial charge separation and transfer kinetics to further transmit photoelectric-signal, resulting in desirable interactive dual-signal with low limit-of-detection (0.21 pM for HPV-18 and 42.92 pM for HPV-16). Thanks to the sophisticated design of differential lateral flow paper-film compound chip and interactive dual-signal amplification strategy, sensitive detection of two HPV genotypes is realized, which provides a promising candidate for home medical intelligent diagnosis.Copyright © 2023 Elsevier B.V. All rights reserved.