离子电流整流（ICR）现象通常发生在纳米孔中，其尺寸与其电双层厚度相当。然而，在某些条件下，微米级的ICR也可以存在于微孔中。在此，构建了填充带电水凝胶的锥形微孔来实现微米级ICR。为了更好地理解微孔ICR，研究了水凝胶空间电荷密度、微孔几何形状、水凝胶填充长度以及电解质浓度和pH值等因素。此外，我们开发了一种基于PEGDA的水凝胶填充微孔感测平台，由于PEGDA的弱电荷，其感测性能得到了提高。 分别以PML / retinoic酸受体α（RARA）融合基因和三磷酸腺苷（ATP）作为模型分析物，在实验中获得了0.1 pM的检测限值。 在均匀和功能性水凝胶填充的微孔中成功实现微米级ICR表明，ICR基础设备的制造，表征和操作可以更加稳健和方便地应用于各个领域。版权所有 © 2023 Elsevier B.V.。保留所有权利。

Ionic current rectification (ICR) phenomena conventionally occurs in nanopores which dimensions are comparable to the thickness of their electrical double layers. However, the microscale ICR in a micropore can also exist under some conditions. Here, the charged hydrogel filled conical micropore was constructed to realize microscale ICR. To better understand the micropore ICR, the influences of space charge density of the hydrogel, micropore geometry, the hydrogel filling length as well as the electrolyte concentration and pH were investigated. Furthermore, we developed a PEGDA-based hydrogel filled micropore sensing platform which sensing performance was enhanced due to the weakly charged PEGDA. The promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARA) fusion genes and adenosine triphosphate (ATP) were respectively used as model analytes and the measured detection limits of 0.1 pM were achieved. The successful realization of microscale ICR in a homogenous and functional hydrogel filled micropore suggests that the fabrication, characterization and operation of ICR based devices can be more robust and facilitated for the wider applications.Copyright © 2023 Elsevier B.V. All rights reserved.