脱髓鞘症在神经系统疾病中有所观察，激励研究人员寻找增强再髓鞘化的成分。我们之前发现，韩国人参中的主要皂苷成分Rb1可以增强髓鞘形成。但是，Rb1或韩国人参是否在体内发挥再髓鞘化的作用尚未研究。小鼠被喂养含0.2％梅花盐的饲料5周，并在3周内每天口服车辆、韩国人参或Rb1，然后返回普通饲料。对脑部进行luxol fast blue（LFB）染色或免疫组织化学分析。将初级神经胶质细胞或星形胶质细胞培养在正常或应激条件下，并给予韩国人参或Rb1处理，以测量髓鞘、内质网（ER）应激、抗氧化剂和白血病抑制因子（LIF）的基因表达。与车辆组相比，KRG或Rb1在第6.5周但不在第8周时增加了髓鞘水平，通过髓体突触粘附蛋白+或谷胱甘肽S-转移酶π+区域进行测量。第5周时，神经胶质前体细胞、星形胶质细胞和微胶质细胞的水平很高，在第5周后减少，但不会因KRG或Rb1发生变化。在初级神经胶质细胞培养中，KRG或Rb1增加了髓鞘基因、ER应激标志物和抗氧化剂的表达。有趣的是，在梅花盐处理下，KRG或Rb1抵消了升高的ER应激标志物。在罗呐酮处理下，Rb1恢复了减少的髓鞘基因表达。在初级星形胶质细胞培养中，KRG或Rb1降低了LIF的表达。韩国人参和Rb1可能通过直接促进髓鞘基因表达来改善体内再髓鞘化阶段的髓鞘再生。© 2022年韩国人参学会。Elsevier B.V.出版服务。

Demyelination has been observed in neurological disorders, motivating researchers to search for components for enhancing remyelination. Previously we found that Rb1, a major ginsenoside in Korean Red Ginseng (KRG), enhances myelin formation. However, it has not been studied whether Rb1 or KRG function in remyelination after demyelination in vivo.Mice were fed 0.2% cuprizone-containing chow for 5 weeks and returned to normal chow with daily oral injection of vehicle, KRG, or Rb1 for 3 weeks. Brain sections were stained with luxol fast blue (LFB) staining or immunohistochemistry. Primary oligodendrocyte or astrocyte cultures were subject to normal or stress condition with KRG or Rb1 treatment to measure gene expressions of myelin, endoplasmic reticulum (ER) stress, antioxidants and leukemia inhibitory factor (LIF).Compared to the vehicle, KRG or Rb1 increased myelin levels at week 6.5 but not 8, when measured by the LFB+ or GST-pi+ area within the corpus callosum. The levels of oligodendrocyte precursor cells, astrocytes, and microglia were high at week 5, and reduced afterwards but not changed by KRG or Rb1. In primary oligodendrocyte cultures, KRG or Rb1 increased expression of myelin genes, ER stress markers, and antioxidants. Interestingly, under cuprizone treatment, elevated ER stress markers were counteracted by KRG or Rb1. Under rotenone treatment, reduced myelin gene expressions were recovered by Rb1. In primary astrocyte cultures, KRG or Rb1 decreased LIF expression.KRG and Rb1 may improve myelin regeneration during the remyelination phase in vivo, potentially by directly promoting myelin gene expression.© 2022 The Korean Society of Ginseng. Publishing services by Elsevier B.V.