使用超声引导静脉注射的方法在小鼠模型中诱导肝转移。
Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection.
发表日期：2023 Mar 15
作者：
Amanda Labora, Hailey Lee, Charlotte Chan, Erin Tabornal, Thuc Le, Khalid Rashid, Evan Abt, Takanobu Yamao, Hanna Mandl, Amanda Creech, Alykhan Premji, Luyi Li, Jason Link, Nanping Wu, Caius Radu, Timothy Donahue
来源：
PHARMACOLOGY & THERAPEUTICS
摘要：
我们在这里介绍一种通过超声引导直接将肿瘤细胞注入门静脉制作肝转移小鼠模型的方案。我们详细说明了动物和细胞准备步骤，以及注射肿瘤细胞的两种技术。其中一种技术是手持自由注射，另一种技术是使用三维打印的原型装置进行辅助注射。最后，我们介绍了生物发光成像进行肿瘤监测的方法。版权所有©2023作者。由Elsevier出版发行，保留所有权利。
Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the other technique is device-assisted using a 3D-printed prototype device. Finally, we describe tumor surveillance with bioluminescent imaging.Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.