这个包含30种颜色的全谱流式细胞术面板是专门开发和优化用于深入分析肿瘤微环境和外周淋巴器官中T细胞免疫表型的。该面板首先可以识别主要的细胞亚群，包括髓系细胞、B细胞、NKT细胞、γδ T细胞、CD4+ T细胞和CD8+ T细胞。对于CD4+ T细胞或CD8+ T细胞，该面板包括标记物，以进一步描述其激活状态（如CD44、CD62L、CD69、Ki67、CD127、KLRG1和CXCR3）、共刺激/共抑制分子（如ICOS、OX-40、PD-1、LAG3、TIM-3、CTLA-4 和TIGIT）、促炎症/抑炎症细胞因子（如IFN-γ、TNF-α和IL-10）以及细胞毒素（如穿孔素、谷氨酰胺酶B和CD107a）。该面板已经在B16-F10小鼠黑色素瘤模型中的肿瘤浸润T细胞和相应的脾T细胞上进行了测试。版权所有©2023 Elsevier B.V. 发布。

This 30-color full spectrum flow cytometry panel was developed and optimized for in-depth analysis T cells immunophenotype in tumor microenvironment and peripheral lymphoid organs. The panel presented here first identify the main cell subsets including myeloid cells, B cells, NKT cells, γδ T cells, CD4+ T cells and CD8+ T cells. For CD4+ T cells or CD8+ T cells, the panel includes markers for further characterization by including a selection of activation status(CD44, CD62L, CD69, Ki67, CD127, KLRG1 and CXCR3), costimulatory/co-inhibitory molecules (ICOS, OX-40, PD-1, LAG3, TIM-3, CTLA-4 and TIGIT), pro-inflammatory/anti-inflammatory cytokines (IFN-γ, TNF-α and IL-10) and cytotoxic molecules (Perforin, Granzymes B and CD107a). The panel has been tested on the tumor infiltrating T cells and corresponding spleen T cells in B16-F10 murine melanoma models.Copyright © 2023. Published by Elsevier B.V.