在学术环境中进行非侵入性多种生物标志物检测的标准操作程序(SOPs)：对RENOVATE研究协议文献的关键评价。
Standard Operating Procedures (SOPs) for non-invasive multiple biomarkers detection in an academic setting: a critical review of the literature for the RENOVATE study protocol.
发表日期：2023 Mar 15
作者：
Martina Dameri, Gabriella Cirmena, Francesco Ravera, Lorenzo Ferrando, Paola Cuccarolo, Mario Stabile, Giuseppe Nicolò Fanelli, Pier Vitale Nuzzo, Massimo Calabrese, Alberto Tagliafico, Alberto Ballestrero, Gabriele Zoppoli
来源：
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
摘要：
液体活检具有极大的潜力，可以显著改变临床实践，为癌症诊断和治疗开辟一种新的非侵入性方法。液体活检在临床实践中实施的限制之一是缺乏共享且可重复的标准操作规程（SOPs），用于样本收集、处理和存储。在这里，我们提出了一份文献的关键评论，重点关注可用的SOPs，以指导液体活检管理的研究设定，并描述我们实验室在前瞻性临床转化试验（RENOVATE，NCT04781062）的背景下开发和应用的SOPs。本文的主要目的是解决常见问题，促进实现跨实验室共享的协议，以优化血液和尿液样本的预分析处理。据我们所知，这项工作是为数不多的关于处理液体活检试验级程序的最新、免费的综合报告之一（Copyright © 2023. Elsevier B.V.） 。
Liquid biopsy has the potential to drastically change clinical practice, paving the way to a novel non-invasive approach for cancer diagnosis and treatment. One of the limitations for the implementation of liquid biopsy in clinical practice is the lack of shared and reproducible standard operating procedures (SOPs) for sample collection, processing and storage. Here, we present a critical review of the literature focusing on the available SOPs to guide liquid biopsy management in research settings and describe SOPs that our laboratory developed and employed in the context of a prospective clinical-translational trial (RENOVATE, NCT04781062). The main aim of this manuscript is to address common issues, towards the implementation of interlaboratory shared protocols for optimized preanalytical handling of blood and urine samples. To our knowledge, this work is one of the few up-to-date, freely available comprehensive reports on trial-level procedures for the handling of liquid biopsy.Copyright © 2023. Published by Elsevier B.V.