DNA修复在癌症的发展、进展、预后和治疗反应中是一个必要的因素。我们利用基于甲酰基嘧啶DNA糖苷酶（Fpg）修饰的彗星检测法，开发了一种细胞修复测定法，以评估DNA修复动力学。在外周血单个核细胞（PBMCs）和8种细胞系中，通过暴露于Ro19-8022加可见光或溴酸钾（KBrO3）来诱导DNA损伤。Ro19-8022加光引起的初始损伤程度在细胞系之间存在差异，这显然与修复速率有关。然而，KBrO3引起的DNA损伤程度在不同细胞类型之间变化较小，因此我们使用该试剂来研究DNA修复的动力学。我们发现有一个约60分钟的早期阶段，快速清除Fpg敏感位点，接下来的7小时清除速度较慢。总之，通过使用KBrO3调整T0的初始损伤程度，可以实现相等水平，从而准确分析曝光后第一小时的细胞DNA修复动力学。©2023年作者。

DNA repair is an essential agent in cancer development, progression, prognosis, and response to therapy. We have adapted a cellular repair assay based on the formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay to assess DNA repair kinetics. The removal of oxidized nucleobases over time (0-480 min) was analyzed in peripheral blood mononuclear cells (PBMCs) and 8 cell lines. DNA damage was induced by exposure to either Ro19-8022 plus visible light or potassium bromate (KBrO3). The initial amount of damage induced by Ro 19-8022 plus light varied between cell lines, and this was apparently associated with the rate of repair. However, the amount of DNA damage induced by KBrO3 varied less between cell types, so we used this agent to study the kinetics of DNA repair. We found an early phase of ca. 60 min with fast removal of Fpg-sensitive sites, followed by slower removal over the following 7 h. In conclusion, adjusting the initial damage at T0 to an equal level can be achieved by the use of KBrO3, which allows for accurate analysis of subsequent cellular DNA repair kinetics in the first hour after exposure.© 2023. The Author(s).