通过X射线辐照诱导降低Lewis Y抗原的机制。本原始研究于2020年至2022年在中国共和国杭州浙江大学城市学院进行。通过Western blot、共免疫沉淀（CO-IP）、电泳迁移实验以及细胞计数试剂盒-8（CCK8），确认了X射线对A549细胞增殖及其机制的影响。数据使用SPSS 11.5进行分析。X射线辐照后糖基转移酶IV和Lewis Y的表达降低，从而抑制A549肺癌细胞的增殖。辐照引起的脱氧核糖核酸损伤导致多聚腺苷二磷酸核糖化特异性蛋白1（SP1）水平升高，SP1从细胞核转移，降低糖基转移酶IV和Lewis Y的表达。在肺癌放疗中，糖基化发挥了重要作用。

To identify the mechanism of down-regulation of Lewis Y antigen caused by X-ray irradiation.The present original research study was conducted at Zhejiang University City College, Hangzhou, Republic of China, from 2020 to 2022. Western blotting, Co-immunoprecipitation (CO-IP), electrophoretic mobility shift assay and Cell Counting Kit-8 (CCK8) were performed to confirm the effect of X-ray irradiation on A549 cell proliferation and its mechanism. Data was analysed using Statistical Package for Social Sciences (SPSS) 11.5.The expressions of fucosyltransferase IV and Lewis Y were decreased after X-ray irradiation, thus inhibiting the proliferation of A549 lung cancer cells. Deoxyribonucleic acid damage caused by the irradiation caused higher level of poly- adenosinediphosphate-ribosylated Specific Protein 1(SP1), and translocation of SP1 from the nucleus, decreasing the expression of fucosyltransferase IV and Lewis Y.There was a significant role of glycosylation in radiation therapy for lung cancer.