背景：肿瘤抗原（CTA）是一类在许多癌症类型中经常过度表达的免疫刺激性抗原。CTA的使用作为免疫治疗靶点已在不同癌症中广泛研究，包括黑色素瘤、血液恶性肿瘤和结直肠癌。研究表明，CTA的表达受到其表观遗传调控的影响，如甲基化状态。然而，有关CTA甲基化状态的报告存在矛盾。CTA的一般甲基化特征，特别是在结直肠癌中的特征，仍然不明确。 目的：确定我们结直肠癌患者中所选CTA的甲基化特征。 方法：使用Infinium人类甲基化450K微阵列芯片对总共54对结直肠癌样本的DNA甲基化特征进行了分析。 结果：我们发现大多数CTA为低甲基化状态，而CCNA1和TMEM108基因是少数几个高甲基化的CTA之一。 结论：总体而言，我们的简要报告已经显示了结直肠癌中200多个CTA的甲基化特征，这可以用于进一步精细化免疫治疗靶点。

Background: Cancer testis antigens (CTAs) are a class of immune-stimulating antigens often overexpressed in many types of cancers. The usage of the CTAs as immunotherapy targets have been widely investigated in different cancers including melanoma, hematological malignancies, and colorectal cancer. Studies have indicated that the epigenetic regulation of the CTAs such as the methylation status may affect the expression of the CTAs. However, the report on the methylation status of the CTAs is conflicting. The general methylation profile of the CTAs, especially in colorectal cancer, is still elusive. Objective: To determine the methylation profile of the selected CTAs in our colorectal cancer patients. Methods: A total of 54 pairs of colorectal cancer samples were subjected to DNA methylation profiling using the Infinium Human Methylation 450K bead chip. Results: We found that most of the CTAs were hypomethylated, and CCNA1 and TMEM108 genes were among the few CTAs that were hypermethylated. Conclusion: Overall, our brief report has managed to show the overall methylation profile in over the 200 CTAs in colorectal cancer and this could be used for further refining any immunotherapy targets.