本研究旨在首次基于新颖且选择性的分子印迹聚合物（MIP）方法，开发一种电化学分析方法来确定一种重要的抗肿瘤药物——托泊替康（TPT）。该MIP是采用电聚合方法，在一种由壳聚糖稳定的金纳米粒子装饰的金属有机骨架上，以TPT为模板分子，以吡咯（Pyr）为功能单体合成的。通过各种物理技术对材料形态和物理特性进行了表征。采用循环伏安法（CV）、电化学阻抗光谱法（EIS）和差分脉冲伏安法（DPV）评估了所得传感器的分析特性。在所有表征和优化实验条件后，评估了MIP-Au-CH @ MOF-5和NIP-Au-CH @ MOF-5在玻碳电极（GCE）上的性能。MIP-Au-CH@MOF-5/GCE表现出广泛的线性响应范围（0.4-70.0 nM）和低检测限（LOD）（0.298 nM）。所开发的传感器在人体血浆和鼻液样品中表现出良好的回收率，分别为94.41-106.16％和95.1-107.0％，验证了其在未来现场监测真实样品中检测TPT的潜力。该方法提供了一种使用MIP方法的不同电化学过程的方法。此外，通过其识别TPT而不被潜在干扰剂干扰的能力，证明了所开发传感器的高灵敏度和选择性。因此，可以推测制备的MIP-Au-CH@MOF-5/GCE可能在许多领域中得到应用，包括公共卫生和食品质量。©2023年作者，独家许可给Springer-Verlag GmbH Austria，该公司是Springer Nature的一部分。

The present study aims to develop an electroanalytical method to determine one of the most significant antineoplastic agents, topotecan (TPT), using a novel and selective molecular imprinted polymer (MIP) method for the first time. The MIP was synthesized using the electropolymerization method using TPT as a template molecule and pyrrole (Pyr) as the functional monomer on a metal-organic framework decorated with chitosan-stabilized gold nanoparticles (Au-CH@MOF-5). The materials' morphological and physical characteristics were characterized using various physical techniques. The analytical characteristics of the obtained sensors were examined by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). After all characterizations and optimizing the experimental conditions, MIP-Au-CH@MOF-5 and NIP-Au-CH@MOF-5 were evaluated on the glassy carbon electrode (GCE). MIP-Au-CH@MOF-5/GCE indicated a wide linear response of 0.4-70.0 nM and a low detection limit (LOD) of 0.298 nM. The developed sensor also showed excellent recovery in human plasma and nasal samples with recoveries of 94.41-106.16 % and 95.1-107.0 %, respectively, confirming its potential for future on-site monitoring of TPT in real samples. This methodology offers a different approach to electroanalytical procedures using MIP methods. Moreover, the high sensitivity and selectivity of the developed sensor were illustrated by the ability to recognize TPT over potentially interfering agents. Hence, it can be speculated that the fabricated MIP-Au-CH@MOF-5/GCE may be utilized in a multitude of areas, including public health and food quality.© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.