斑蛇鱼墨汁多糖（SIP）和其硫酸衍生物（SIP-SII）已被证明具有各种生物活性，但低分子量鱿鱼墨汁多糖（LMWSIPs）的了解甚少。本研究通过酸解法制备了LMWSIPs，并将分子量（Mw）分布在7 kDa到9 kDa、5 kDa到7 kDa和3 kDa到5 kDa范围内的片段分别命名为LMWSIP-1、LMWSIP-2和LMWSIP-3。阐明了LMWSIPs的结构特征，并研究了它们的抗肿瘤、抗氧化和免疫调节活性。结果表明，除了LMWSIP-3外，LMWSIP-1和LMWSIP-2的主要结构与SIP相比没有改变。虽然LMWSIPs和SIP的抗氧化能力没有明显差异，但在降解后，SIP的抗肿瘤和免疫调节活性在一定程度上得到了提高。特别值得注意的是，LMWSIP-2在抗增殖、促进凋亡和抑制肿瘤细胞迁移以及促进脾淋巴细胞增殖方面的活性显著高于SIP和其他降解产物，这对于抗肿瘤药物领域是有前途的。版权所有©2023 Elsevier B.V.发布。

Sepiella maindroni ink polysaccharide (SIP) from the ink of cuttlefish Sepiella maindroni and its sulfated derivative (SIP-SII) have been demonstrated to possess diverse biological activities. But little is known about low molecular weight squid ink polysaccharides (LMWSIPs). In this study, LMWSIPs were prepared by acidolysis, and the fragments with molecular weight (Mw) distribution in the ranges of 7 kDa to 9 kDa, 5 kDa to 7 kDa and 3 kDa to 5 kDa were grouped and named as LMWSIP-1, LMWSIP-2 and LMWSIP-3, respectively. The structural features of LMWSIPs were elucidated, and their anti-tumor, antioxidant and immunomodulatory activities were also studied. The results showed that with the exception of LMWSIP-3, the main structures of LMWSIP-1 and LMWSIP-2 did not change compared with SIP. Though there were no significant differences in the antioxidant capacity between LMWSIPs and SIP, the anti-tumor and immunomodulatory activities of SIP were enhanced to a certain extent after degradation. It is particularly noteworthy that the activities of LMWSIP-2 in anti-proliferation, promoting apoptosis and inhibiting migration of tumor cells as well as promoting the proliferation of spleen lymphocytes were significantly higher than those of SIP and the other degradation products, which is promising in the anti-tumor pharmaceutical field.Copyright © 2023. Published by Elsevier B.V.