非小细胞肺癌是一种由广泛分子改变驱动的异质性疾病。外泌体是各种细胞释放的直径范围为30到150 nm的小囊泡，是肿瘤细胞信息传递的重要介质。外泌体包含蛋白质、脂质以及各种类型的核酸，包括miRNA甚至DNA和RNA。MFI2反义RNA 1 (MFI2-AS1)是一种长链非编码RNA，已知在各种恶性肿瘤中促进细胞增殖、转移和侵袭。使用RNA荧光原位杂交（FISH）染色检测了NSCLC组织中MFI2-AS1的相对表达。采用Transwell迁移和划痕愈合实验分析了细胞迁移和侵袭能力。管型形成用于评估血管生成能力。使用CCK8评估细胞增殖能力。RNA免疫沉淀(RIP)实验证实了MFI2-AS1作为miR-107的竞争性内源性RNA(ceRNA)的作用。使用双荧光素酶报告基因实验鉴定MFI2-miRNA和靶mRNA之间的潜在结合。通过向皮下肿瘤注射外泌体建立动物模型的体内实验。外泌体MFI2-AS1通过海绵化miR-107增加NFAT5的表达，进而激活PI3K / AKT通路。我们发现，MFI2-AS1 / miR-107 / NFAT5轴在外泌体介导的NSCLC进展中起着重要作用，涉及前转移巢穴的形成，并可用作NSCLC转移的血液生物标志物。我们证明，在转移性NSCLC细胞释放的外泌体中上调MFI2-AS1并可转移至HUVECs，促进血管生成和迁移。©2023.作者。

Non-small cell lung cancer is a heterogeneous disease driven by extensive molecular alterations. Exosomes are small vesicles with diameters ranging from 30 to 150 nm released by various cell types and are important mediators of information transmission in tumor cells. Exosomes contain proteins, lipids, and various types of nucleic acids, including miRNAs and even DNA and RNA. MFI2 Antisense RNA 1 (MFI2-AS1) is a long noncoding RNA known to promote cell proliferation, metastasis and invasion in a variety of malignancies.The relative expression of MFI2-AS1 in NSCLC tissues was examined using RNA fluorescence in situ hybridization (FISH) staining. Transwell migration and wound healing assays were used to analyze cell migration and invasion abilities. Tube formation is used to assess angiogenic capacity. CCK8 was used to assess cell proliferation ability. RNA immunoprecipitation (RIP) experiments confirmed that MFI2-AS1 acts as a competing endogenous RNA (ceRNA) for miR-107. Dual-luciferase reporter assays were used to identify potential binding between MFI2-miRNA and target mRNA. In vivo experiments were performed by injecting exosomes into subcutaneous tumors to establish animal models.Exosomal MFI2-AS1 increases NFAT5 expression by sponging miR-107, which in turn activates the PI3K/AKT pathway. We found that the MFI2-AS1/miR-107/NFAT5 axis plays an important role in exosome-mediated NSCLC progression, is involved in pre-metastatic niche formation, and can be used as a blood-based biomarker for NSCLC metastasis.We demonstrate that MFI2-AS1 is upregulated in exosomes secreted by metastatic NSCLC cells and can be transferred to HUVECs, promoting angiogenesis and migration.© 2023. The Author(s).