长期暴露在含高浓度无机砷（NaAsO2）的饮用水中与肝毒性和糖尿病风险的增加有关。奇骏素具有抗氧化和抗炎作用等多种药理学特性。本研究旨在研究奇骏素对由NaAsO2引起的糖尿病和肝毒性的保护作用。随机选择60只8周龄，体重为25±2 g的雄性NMRI小鼠，分为六组。对照组（第1组）口服蒸馏水，第二组口服奇骏素（100毫克/千克，口服），第三组口服NaAsO2（10毫克/千克，口服），第四、五、六组分别口服奇骏素（25、50、100毫克/千克，口服）和NaAsO2（10毫克/千克，口服）。在29天后进行空腹血糖（FBS）测量和葡萄糖耐量试验。小鼠于第31天被牺牲，取血和组织（肝脏和胰腺）样本。然后，研究了血清和组织样本的生化和组织学评估。结果表明，与NaAsO2组相比，奇骏素改善了葡萄糖耐受性，降低了FBS。奇骏素（50和100毫克/千克）改善了接受NaAsO2组的肝功能血清因子（丙氨酸转移酶、天门冬氨酸转移酶和碱性磷酸酶）。此外，奇骏素治疗减轻了由NaAsO2诱导的肝组织中一氧化氮、肿瘤坏死因子α和硫代巴比妥酸反应性物质水平的增加。奇骏素的给药增加了肝脏组织中总硫醇和过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶等酶的活性。此外，奇骏素治疗降低了接受NaAsO2组的Sirtuin 3和核因子kappaB蛋白质含量的增加。奇骏素治疗也减轻了由NaAsO2引起的肝脏和胰腺组织病理学损害。奇骏素通过其抗氧化和抗炎作用对小鼠由NaAsO2引起的肝毒性和糖尿病具有预防作用。版权所有©2023 Elsevier GmbH。保留所有权利。

Chronic exposure to high concentrations of inorganic arsenic (NaAsO2) in drinking water is related to an increase in the risk of liver toxicity and diabetes. Diosmin has various pharmacological properties, including antioxidant and anti-inflammatory properties. This study was designed to investigate the protective effects of diosmin on diabetes and hepatotoxicity caused by NaAsO2.Sixty male 8-week-old NMRI mice, weighing 25 ± 2 g, were randomly selected and put into six groups. The control (Group 1) was treated orally with distilled water, group 2 was treated with diosmin (100 mg/kg, p.o), group 3 received NaAsO2 (10 mg/kg, p.o), and groups 4, 5, 6 received diosmin (25, 50, 100 mg/kg, p.o), respectively and NaAsO2 (10 mg/kg, p.o). After 29 days, fasting blood sugar (FBS) measurement and glucose tolerance test were done. The mice were sacrificed on day 31, and blood and tissue (liver and pancreas) samples were taken. Then, serum and tissue samples were studied for biochemical and histological evaluations.The results demonstrated that diosmin ameliorated glucose intolerance and decreased FBS compared to the NaAsO2 group. Diosmin (50 and 100 mg/kg) improved the serum factors of liver function (alanine aminotransferase, aspartate transaminase, and alkaline phosphatase) in the groups receiving NaAsO2. Moreover, increased levels of nitric oxide, tumor necrosis factor-alpha, and thiobarbituric acid reactive substances in liver tissue induced by NaAsO2 were diminished by diosmin treatment. Administration of diosmin increased total thiol and enzymatic activities of catalase, superoxide dismutase, and glutathione peroxidase in liver tissue. Furthermore, treatment with diosmin reduced the increase in protein amount of Sirtuin 3 and nuclear factor kappa B in the groups receiving NaAsO2. Also, the liver and pancreas histological lesions induced by NaAsO2 were attenuated by diosmin treatment.Diosmin has a preventive effect against hepatotoxicity and diabetes induced by NaAsO2 in mice through its antioxidant and anti-inflammatory properties.Copyright © 2023 Elsevier GmbH. All rights reserved.