卵巢组织冷冻保存是一种有效的保护青春期女性癌症患者生育能力的策略，因为她们的肿瘤治疗不能延迟。本研究探讨了丝素作为抗氧化剂对小鼠卵巢组织冷冻保存和解冻的影响。小鼠卵巢组织在含有0.5%或1%丝素（重量/体积）和0.1 mM褪黑激素的培养基中冷冻保存和解冻，然后观察卵泡形态。测定了抗氧化酶的水平，包括谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、总超氧化物歧化酶（T-SOD）、总抗氧化能力（T-AOC）和过氧化氢酶（CAT）。此外，还测试了一氧化氮（NO）、丙二醛（MDA）和乳酸脱氢酶（LDH）的水平。此外，还测定了凋亡相关蛋白Bcl-2和Bax的水平。我们的研究结果表明，1%丝素可以保持卵泡形态，抑制凋亡，降低MDA和NO水平，并增加内源性抗氧化酶水平，对LDH水平无显著影响。此外，这些效应可能与磷脂酰肌醇-3-激酶（PI3K）/蛋白激酶B（AKT）/哺乳动物雷帕霉素靶（mTOR）信号通路的激活有关，这也得到了PI3K、p-AKT和mTOR水平增加的证实。这些发现表明，1%丝素可以通过PI3K/AKT/mTOR信号通路减少氧化应激，保护卵巢组织在冷冻保存和解冻过程中的作用。版权所有©2023 Elsevier Inc.发表。

Ovarian tissue cryopreservation is an effective fertility protective strategy for preadolescent female cancer patients, whose tumor treatment cannot be delayed. In the present study, the effects of sericin, as an antioxidant, on mice ovarian tissue freezing and thawing were investigated. Mice ovarian tissues were cryopreserved and thawed in medium containing 0.5% or 1%sericin (w/v), and 0.1 mM melatonin. Then, the follicular morphology was observed. The levels of antioxidant enzymes were determined, including glutathione (GSH), glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) and catalase (CAT). Moreover, the levels of nitric oxide (NO), malondialdehyde (MDA) and lactate dehydrogenase (LDH) were also tested. Besides, apoptosis-related proteins Bcl-2 and Bax were determined. Our results showed that 1% sericin maintained follicular morphology, inhibited apoptosis, decreased MDA and NO levels, and boosted endogenous antioxidant enzyme levels, while had no significant effect on LDH levels. Furthermore, these effects may be related with the activation of the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of Rapamycin (mTOR) signaling pathway, as demonstrated by increased PI3K, p-AKT and mTOR levels. These findings demonstrate that 1% sericin may reduce oxidative stress and protect ovarian tissues during freezing and thawing via PI3K/AKT/mTOR signaling pathway.Copyright © 2023. Published by Elsevier Inc.