通过使用聚乙二醇（PEG）和多肽功能化的低污染感应界面的构建，提出了一种用于电化学测定乳腺癌典型生物标志物——人表皮增生因子受体2（HER2）的简单策略。该HER2生物传感器基于经过导电聚合物聚（3,4-乙烯二氧噻吩）（PEDOT）和金纳米颗粒（AuNPs）改性的电极作为感测基板开发，接着固定具有识别和抗污染双重性质的抗污染PEG和多肽。由于PEG和多肽的联合抗污染效果以及多肽对目标HER2的特异性识别能力，开发的电化学生物传感器在复杂的生物液体（如人血液和血清）中表现出强大的抗污染性能，并且能够在非常广泛的线性范围内（1.0 pg mL-1 至 1.0 μg mL-1）测定目标HER2，具有极低的检测限（0.44 pg mL-1）。两种抗污染生物材料的组合（PEG和多肽）为开发适用于复杂生物环境实际检测的低污染感测平台提供了有效的策略。版权所有© 2023 Elsevier B.V.。

A simple tactic for electrochemical determination of a typical biomarker for breast cancer, human epidermal growth factor receptor 2 (HER2), was presented via the construction of a low fouling sensing interface functionalized with polyethylene glycol (PEG) and peptide. The HER2 biosensor was developed based on an electrode modified by the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and Au nanoparticles (AuNPs) as the sensing substrate, and followed by the immobilization of an antifouling PEG and a peptide with both recognizing and antifouling properties. Thanks to the combined antifouling effect of the PEG and peptide, and the specific recognizing ability of the peptide to the target HER2, the developed electrochemical biosensor exhibited strong antifouling performances in complex biofluids, such as human blood and serum, and it was capable of assaying target HER2 within a very wide linear range (1.0 pg mL-1 to 1.0 μg mL-1), with an ultralow limit of detection (0.44 pg mL-1). The combination of two kinds of antifouling biomaterials (PEG and peptide) offered an effective strategy for the development of low fouling sensing platforms suitable for practical assay in complex biotic environments.Copyright © 2023 Elsevier B.V. All rights reserved.