开发了一种以Fe掺杂的Bi2O2S纳米片光阳极为基础的光电生物监测平台，使用仿生铁氰基纳米粒子（PB）催化的生物催化沉淀策略用于前列腺特异性抗原（PSA）的检测。首先，在目标PSA存在的情况下，通过静电吸附得到的信号探针PB-mAb2被固定在微孔板上，外源性过氧化氢促进大量羟基自由基通过PB催化，氧化4-氯-1-萘酚（4-CN）生成4-氯-己二烯酮苯（4-CD）。生成的4-CD具有强烈的低导电特性，可以破裂高光电活性Fe-Bi2O2S光阳极的光电流。该分割孵化反应可用于高产量和低成本的快速检测。基于光电传感平台和仿生催化沉淀反应的传感器实现了0.1-100ng/mL动态响应范围和34.2pg/mL的检测限。同样重要的是，与商用ELISA试剂盒相比，该传感器在实际样品检测方面也表现出良好的潜力。总之，这项工作通过多功能的仿生催化策略和高光电活性的光阳极耦合，为开发敏感的生物传感器提供了新方案。 版权所有©2023 Elsevier B.V.。保留所有权利。

A Fe-loaded Bi2O2S nanosheet photoanode serving as photoelectric biomonitoring platform for the detection of prostate-specific antigen (PSA) using biologically inspired prussian nanoparticle (PB)-catalyzed biocatalytic precipitation strategy was developed. Primarily, the signal probe PB-mAb2 obtained by electrostatic adsorption was immobilized on a microplate in the presence of target PSA, and 4-chloro-1-naphthol (4-CN) was oxidized to benzo-4-chloro-hexadienone (4-CD) with the assistance of exogenous hydrogen peroxide, which was generated by a large number of hydroxyl radicals catalyzed by PB. The generated 4-CD showed strongly low conductivity characteristics to burst the photocurrent of highly photoactive Fe-Bi2O2S photoanode. The split incubation reaction could be suitable for high volume and low-cost rapid detection. A dynamic response range of 0.1-100 ng mL-1 with a limit of detection of 34.2 pg mL-1 was achieved with the sensor based on a photoelectric sensing platform and a biomimetic catalytic precipitation reaction. Equally important, the sensor also showed good potential in the detection of real samples compared to commercially available ELISA kits. In conclusion, this work provides a fresh scheme for the development of sensitive biosensors through a bio-inspired catalytic strategy of versatility and a photoanode coupling with high photoelectric activity.Copyright © 2023 Elsevier B.V. All rights reserved.