由于诊断方法低效，炎症性肠病（IBD）通常会进展为严重的并发症，包括癌症。高效的代谢指纹提取和识别对于疾病监测非常重要。在本项工作中，我们通过一个简单的单步组装过程合成了一种掺杂了石墨化碳的层状钛纳米片（2D-GC-mTNS），用于辅助激光解吸电离质谱法（LDI-MS）进行代谢产物分析。基于石墨化碳和介孔钛材料的协同效应，2D-GC-mTNS表现出良好的提取能力，包括高灵敏度（< 1 fmol µL-1）和出色的代谢物重复性。共从数百个原始尿样（包括IBD患者和健康对照组）中收集了996个指纹光谱，每个光谱都包含1220 m/z的代谢产物特征。进一步建立了诊断模型，能够精确区分患者和健康对照组，发现队列和验证队列的曲线下面积值分别高达0.972和0.981。2D-GC-mTNS促进了LDI-MS逐渐接近临床应用，具有快速速度，最小样品消耗和无需样品前处理的优点。在线版本包含补充材料，可在10.1007/s43657-022-00055-0处获取。© 2022年国际人类表型研究所（上海）。

Due to inefficient diagnostic methods, inflammatory bowel disease (IBD) normally progresses into severe complications including cancer. Highly efficient extraction and identification of metabolic fingerprints are of significance for disease surveillance. In this work, we synthesized a layered titania nanosheet doped with graphitized carbon (2D-GC-mTNS) through a simple one-step assembly process for assisting laser desorption ionization mass spectrometry (LDI-MS) for metabolite analysis. Based on the synergistic effect of graphitized carbon and mesoporous titania, 2D-GC-mTNS exhibits good extraction ability including high sensitivity (< 1 fmol µL-1) and great repeatability toward metabolites. A total of 996 fingerprint spectra were collected from hundreds of native urine samples (including IBD patients and healthy controls), each of which contained 1220 m/z metabolite features. Diagnostic model was further established for precise discrimination of patients from healthy controls, with high area under the curve value of 0.972 and 0.981 toward discovery cohort and validation cohort, respectively. The 2D-GC-mTNS promotes LDI-MS to be close to clinical application, with rapid speed, minimum sample consumption and free of sample pretreatment.The online version contains supplementary material available at 10.1007/s43657-022-00055-0.© International Human Phenome Institutes (Shanghai) 2022.