子宫内膜腺肌症是一种良性妇科疾病，其病因至今未明。Hippo信号通路在体内高度保守，并与子宫内膜异位症和各种癌症相关。我们的研究旨在探讨与Hippo信号通路相关的蛋白在患有和没有子宫内膜腺肌症的小鼠子宫中的表达情况，并寻求Hippo信号通路与子宫内膜腺肌症细胞迁移、侵袭、增殖和凋亡之间的关系。在患有子宫内膜腺肌症的小鼠中观察到Hippo信号通路失活和EMT相关蛋白异常表达。在体外，YAP抑制剂verteporfin可抑制Ishikawa细胞的增殖和迁移，并促进凋亡，同时抑制EMT过程。此外，腹腔注射verteporfin可抑制子宫内膜腺肌症小鼠子宫内细胞的EMT过程和增殖，并促进凋亡。这表明Hippo信号通路参与了子宫内膜腺肌症细胞的EMT、增殖和凋亡。总之，这些结果表明Hippo信号通路可能通过调节EMT、增殖和凋亡来参与子宫内膜腺肌症的发展，从而为治疗子宫内膜腺肌症提供了潜在的治疗靶点。 ©2023. 作者。

Adenomyosis is a benign gynecological disease. The pathogenesis of adenomyosis is still unclear. The Hippo signaling pathway is highly conserved in vivo and associated with endometriosis and various cancers. Our objective was to study the expression of Hippo signaling pathway-related proteins in the uterus of mice with and without adenomyosis. We also sought to determine the relationship between the Hippo signaling pathway and cell migration, invasion, proliferation, and apoptosis in adenomyosis. The inactivation of Hippo signaling pathway and abnormal expression of EMT-related proteins were observed in mice with adenomyosis. In vitro, the YAP inhibitor verteporfin can inhibit the proliferation and migration of Ishikawa cells and promote apoptosis, while inhibiting the EMT process. In addition, intraperitoneal injection of verteporfin inhibits EMT process and proliferation and promotes apoptosis of cells in the uterus of adenomyosis mice. It suggests that the Hippo signaling pathway participates in the EMT, proliferation, and apoptosis of cells in adenomyosis. In conclusion, these results suggest that Hippo signaling pathway may be involved in the development of adenomyosis by regulating EMT, proliferation, and apoptosis of cells, which provide a potential target for the treatment of adenomyosis.© 2023. The Author(s).