一个24岁的男子患有T细胞急性淋巴细胞白血病中枢神经系统（CNS）侵犯，接受了兄弟供体异基因干细胞移植（allo-SCT）。他在移植后早期出现了孤立的CNS复发，而高剂量全身化疗，腰髓内（IT）三联输注和IT供体淋巴细胞输注（DLI）都表现出了有效性。我们进行了未曾报道的IT脐带血来源的CAR-NK（CD7靶向）细胞输注。输注后，细胞因子检测显示脑脊液中干扰素-γ、白细胞介素-6和白细胞介素-8增加。他在短时间内发生高热、头痛、恶心、呕吐和脊髓断裂并失禁，但眼睑下垂和视力模糊完全好转。骨髓仍然保持着完全缓解和完全供体嵌合状态，在IT CAR-NK细胞输注后9个月以上。总之，IT CAR-NK细胞输注是一种潜在可行且有效的选项，适用于CNS复发的患者，具有有限的神经毒性。©2023. 作者（们）。

A 24-year-old man with central nervous system (CNS) involvement of T-cell lineage acute lymphoblastic leukemia received sibling allogeneic stem cell transplantation (allo-SCT). He developed isolated CNS relapse early post-SCT, while high-dose systemic chemotherapy, intrathecal (IT) triple infusion and IT donor lymphocytes infusion (DLI) all demonstrated effectiveness. We performed IT umbilical cord blood-derived CAR-NK (target CD7) cells infusion, which was not previously reported. After infusion, detection of cytokines revealed that interferon-γ, interleukin-6 and interleukin-8 increased in CSF. He developed high fever, headache, nausea, vomiting and a spinal cord transection with incontinence in a short time, whereas the ptosis and blurred vision improved completely. The bone marrow remained encouragingly complete remission and complete donor chimerism over 9 months after IT CAR-NK cells infusion. In conclusion, IT CAR-NK cells infusion is a potentially feasible and effective option for patients with CNS relapse, with limited neurological toxicity.© 2023. The Author(s).