克服卵巢组织移植后癌细胞复发的一种方法是使用去细胞化的组织作为支架，不含任何细胞成分。这些无细胞的支架可以用不同类型的干细胞进行卵巢修复。卵泡母细胞干细胞（OSCs）、腹膜干细胞（PMSCs）和骨髓间充质干细胞（BMSCs）分别被种植在人类去细胞化的卵巢组织中，形成四组：支架+OSC（SO）、支架+OSC+PMSCs（SOP）、支架+OSC+BMSCs（SOB）和支架+OSC+PMSCs+BMSCs（SOPB）。所产生的移植物被植入摘除卵巢的NMRI小鼠的腹膜下，成为人造卵巢（AO）。通过qRT-PCR测量AO中Vegf、CD34、Gdf9、Zp3、Ddx4、Amh和Lhr基因的表达。此外，采用组织学技术检测抗GFP、PCNA、VEGF、GDF9、ZP3和AMH蛋白。H＆E染色显示所有组中都有卵泡样结构；在SOP和SOB组中，这些结构的数量最多。在SO组中，观察到到卵母细胞和颗粒细胞的分化能力。在SOP组中，存在内皮细胞、卵母细胞、生殖细胞和颗粒细胞样细胞，SOPB组的血管生成能力更强。然而，SOPB组的血管生成能力和类黄体腺细胞样细胞的分化能力更强。虽然所有组的AMH水平没有显著差异，但是在SOPB组中雌二醇水平显著更高。OSC+PMSCs和OSC+BMSCs的融合更有利于卵母发育。©2023年，作者。

One way to overcome the recurrence of cancer cells following ovarian tissue transplantation is to use decellularized tissues as a scaffold that does not have any cellular components. These cell-free scaffolds can be seeded with different type of stem cells for ovarian restoration.OSCs, PMSCs and BMSCs (oogonial, peritoneal and bone marrow mesenchymal stem cells, respectively) were seeded into human decellularized ovarian tissue as 4 groups: Scaffold + OSCs (SO), Scaffold + OSC + PMSCs (SOP), Scaffold + OSC + BMSCs (SOB) and Scaffold + OSC + PMSCs + BMSCs (SOPB). The produced grafts were transplanted into the sub-peritoneal space of ovariectomized NMRI mice as artificial ovary (AO). The expression of Vegf, CD34, Gdf9, Zp3, Ddx4, Amh and Lhr genes in AOs were measured by qRT-PCR. Also, histotechniques were considered to detect the anti GFP, PCNA, VEGF, GDF9, ZP3 and AMH proteins.H & E staining showed follicle-like structures in all groups; the number of these structures, in the SOP and SOB groups, were the highest. In SO group, differentiation ability to oocyte and granulosa cells was observed. Endothelial, oocyte, germ, and granulosa cell-like cells were specially seen in SOP and angiogenesis capability was more in SOB group. However, angiogenesis ability and differentiation to theca cell-like cells were more often in SOPB group. While none of the groups showed a significant difference in AMH level, estradiol levels were significantly higher in SOPB group.Integration of OSCs + PMSCs and those OSCs + BMSCs were more conducive to oogenesis.© 2023. The Author(s).