对于癌症的诊断和病理分析来说，同时监测多个低丰度信使RNA（mRNA）的时空分布非常重要。然而，在亚细胞水平上高效同时监测并跟踪多种mRNA的位置仍然是临床上的挑战。在这里，我们提出了利用聚A介导的双色粘性探针，可以同时成像两种细胞内mRNA生物标志物。两种荧光DNA，分别专门针对GalNac-T mRNA和c-Myc mRNA，并通过高效的聚腺嘌呤（polyA）连接到金纳米颗粒（AuNPs）上。通过调节聚A的长度，可以精确地设计AuNPs表面DNA的间距和密度。与传统的蒂欧-DNA修饰纳米探针相比，粘性探针的均匀性、检测灵敏度和响应动力学得到了大大提高，这使得mRNA的实时成像效率得以提高。由于具有粘性端设计，荧光DNA可以在与靶mRNA结合后动态跟踪mRNA，实现了亚细胞内mRNA的时空监测。与单个目标mRNA成像模式相比，多目标成像模式可以更准确地诊断癌症。此外，所提出的聚A介导的双色粘性探针具有良好的细胞入侵效率和低毒性，并且具有低成本和简单的组装工艺，可为活细胞中多个目标成像提供重要工具。

Spatiotemporal monitoring of multiple low-abundance messenger RNAs (mRNAs) is vitally important for the diagnosis and pathologic analysis of cancer. However, it remains a clinical challenge to monitor and track multiple mRNAs location simultaneously in situ at subcellular level with high efficiency. Herein, we proposed polyA-mediated dual-color sticky flares for simultaneous imaging of two kinds of intracellular mRNA biomarkers. Two kinds of fluorescent DNA specific for GalNac-T mRNA and c-Myc mRNA were functionalized onto gold nanoparticles (AuNPs) through efficient polyadenine (polyA) attachment. By tuning polyA length, the lateral spacing and densities of DNA on AuNPs could be precisely engineered. Compared to the traditional thio-DNA-modified nanoprobes, the uniformity, detection sensitivity, and response kinetics of sticky flares were greatly improved, which enables live-cell imaging of mRNAs with enhanced efficiency. With a sticky-end design, the fluorescent DNA could dynamically trace mRNAs after binding with target mRNAs, which realized spatiotemporal monitoring of subcellular mRNAs in situ. Compared to one target mRNA imaging mode, the multiple target imaging mode allows more accurate diagnosis of cancer. Furthermore, the proposed polyA-mediated dual-color sticky flares exhibit excellent cell entry efficiency and low cytotoxicity with a low-cost and simple assembling process, which provide a pivotal tool for multiple targets imaging in living cells.