慢性接触二氧化硅与诱发炎性反应和氧化应激机制有关。维生素D对生物活动尤其是呼吸系统疾病具有多种益处。在这项研究中，将20只雄性Wistar大鼠随机分成四组（每组5只）：第1组接受生理盐水（阴性对照）；第2组单次肺泡内注射硅（阳性对照）；第3组在90天内每天同时给予单次硅注射和维生素D（20 mg/kg/天）；第4组在90天内每天接受维生素D。硅显著增加了血清和肺脏总氧化状态（TOS）。同时，硅降低了血清和肺部总抗氧化能力（TAC）、GSH和肿瘤坏死因子-α（TNF-a）。维生素D治疗有意地逆转了氧化应激、抗氧化状态和炎症反应。此外，维生素D改善了硅引起的组织病理变化。这些发现表明，维生素D对硅致肺损伤具有保护作用。看来，维生素D有潜在用于硅致肺损伤的治疗对象。© 2023职业安全卫生研究所。

Chronic exposure to silica is related with the provocation of an inflammatory response and oxidative stress mechanism. Vitamin D has multiple benefits in biological activities particularly respiratory system disease.In this research, 20 male Wistar rats were randomly allocated into four groups (5 rats /group) as follow: Group1 received saline as (negative control) group. The group 2 received a single IT instillation of silica (positive control) group; the group 3 was co-administrated with single IT silica and Vitamin D (20 mg/kg/day) daily for a period of 90 days. The rats of group 4 received Vitamin D daily for a period of 90 days.Silica significantly increased serum and lung total Oxidant Status (TOS). Meanwhile, silica reduced serum and lung total antioxidant capacity (TAC), GSH and tumor necrosis factor-α (TNF-a). Vitamin D treatment meaningfully reversed oxidative stress, antioxidants status and inflammatory response. Also, Vitamin D improved histopathological changes caused by silica.These findings indicate that Vitamin D exerts protective effects against silica-induced lung injury. It seems that Vitamin D has potential use as a therapeutic object for silica induced lung injure.© 2023 Occupational Safety and Health Research Institute.