具有可调节、功能化和可用于可视化过程的分子探针对于理解和操作生物系统非常有价值。得益于其独特的聚集诱导发光特性、可调结构和对外部刺激的响应，四苯基乙烯（TPE）的几何顺反异构体成为实现这些任务的有吸引力的候选物。本篇小型综述着重介绍了与分子识别单元功能化的TPE衍生物的顺、反异构体有关的荧光检测、生物成像和癌症治疗。总结了分子几何对荧光性质、靶向结合能力和生物活性的影响。讨论了通过体外和体内切换分子构型以及生物活性的可行性。最后，针对基于TPE立体异构体的靶向感测和成像引导生物过程的调控，讨论了未来的发展和挑战。© 2023 Wiley-VCH GmbH.

Molecular probes which can be modulated, functionalized and used to visualize the processes are highly desirable for understanding and manipulating biological systems. Geometric cis and trans isomers of tetraphenylethene (TPE) emerge as attractive candidates to fulfill these tasks thanks to the unique aggregation-induced emission properties, tailorable structures, and responsiveness to external stimuli. This minireview focuses on cis and trans isomers of TPE derivatives that are functionalized with molecular recognition units for fluorescence detection, bioimaging and cancer therapy. The effects of molecular geometry on fluorescence property, target binding ability and biological activity are summarized. The feasibility to in vitro and in vivo switch molecular configuration and thus bio-activity is discussed. Finally, the future development and challenges are discussed in view of TPE-based stereoisomers for targeted sensing and imaging-guided modulation of biological processes.© 2023 Wiley-VCH GmbH.