恶性肿瘤中经常观察到退化的组织，但它们没有被分析。我们调查了核漂移和坏死样本是否可用于遗传分析以扩大样本池。总共从小细胞癌和淋巴瘤FFPE组织块中提取了81个样本，并分为三个组：33个具有良好保留肿瘤形态的材料，31个核漂移样本和17个坏死样本。比较了DNA和RNA完整性指数、具有>200核苷酸的RNA片段百分比和下一代测序的质量指标。DNA质量在具有良好保留形态的材料和核漂移材料之间没有显著差异，而坏死样本的质量较差。 RNA质量按照以下顺序降低：具有良好保留形态的材料>核漂移>坏死。序列测量在核漂移样本和具有良好保留形态的材料之间没有显著差异，并检测到可靠的变异体。从切除手术中提取的坏死样本显示测序失败，并且显示出显著较少的对准读数和变异体。然而，变异基因频率在组中没有差异。我们发现核漂移样本中的DNA，尤其是在活检中，可用于遗传分析。而且，在评估肿瘤内容时应考虑退化的非肿瘤细胞，以避免误解变异基因频率。

Degenerated tissues are frequently observed in malignant tumors, but are not analyzed. We investigated whether nuclear streaming and necrosis samples could be used for genetic analysis to expand the sample pool. A total of 81 samples were extracted from small cell carcinoma and lymphoma FFPE tissue blocks and classified into three histological cohorts: 33 materials with well-preserved tumor morphology, 31 nuclear streaming samples, and 17 necrosis samples. DNA and RNA integrity numbers, percentage of RNA fragments with >200 nucleotides, and next-generation sequencing quality metrics were compared among the cohorts. DNA quality did not significantly differ between nuclear streaming materials and materials with well-preserved morphology, whereas that of the necrosis samples was inferior. RNA quality decreased in the following order: materials with well-preserved morphology > nuclear streaming > necrosis. The sequencing metrics did not differ significantly between the nuclear streaming samples and materials with well-preserved morphology, and reliable variants were detected. The necrosis samples extracted from resections exhibited sequencing failure and showed significantly fewer on-target aligned reads and variants. However, variant allele frequency did not differ among the cohorts. We revelated that DNA in nuclear streaming samples, especially within biopsies, could be used for genetic analysis. Moreover, degenerated non-tumor cells should be counted when evaluating tumor content to avoid misinterpreting the variant allele frequency.