植物介导的金属纳米颗粒已被报道在生物科学领域中应用广泛。在本研究中，我们提出了土本香 (Polianthes tuberosa) 花作为还原和稳定剂，用于合成银纳米颗粒 (PTAgNPs)。我们仅通过使用紫外-可见光谱，傅里叶变换红外光谱 (FTIR)，扫描电子显微镜 (SEM)，X射线衍射 (XRD)，原子力显微镜，电荷-电位分布规律以及透射电子显微镜 (TEM) 研究了PTAgNPs的特性。在生物实验中，我们调查了银纳米颗粒在A431细胞系中的抗菌和抗癌作用。PTAgNPs在大肠杆菌和金黄色葡萄球菌中表现出剂量依赖性活性，表明AgNPs具有杀菌性。PTAgNPs展示了在A431细胞系中的剂量依赖性毒性，IC50为54.56µg/mL，在流式细胞仪分析中显示出阻止S期细胞生长。 COMET试验表明，在受处理的细胞系中具有39.9%和18.15%的DNA损伤严重程度和尾巴长度。荧光染色研究表明PTAgNPs可导致活性氧物种 (ROS) 并引发凋亡。这项研究证明，合成的银纳米颗粒对于抑制黑色素瘤细胞和其他形式的皮肤癌有显着的作用。结果表明，这些颗粒能够在恶性肿瘤细胞中引起凋亡或细胞死亡。这表明，它们可能可用于治疗皮肤癌，而不会损害正常组织。

Plant-mediated metallic nanoparticles have beenreported for a diversified range of applications in biological sciences. In the present study, we propose the Polianthes tuberosa flower as a reducing and stabilizing agent for the synthesis of silver nanoparticles (PTAgNPs). The PTAgNPs were exclusively characterized using UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), atomic force microscopy, zeta potential, and transmission electron microscopy (TEM) studies. In a biological assay, we investigated the antibacterial and anticancer activity of silver nanoparticles in the A431 cell line. The PTAgNPs demonstrated a dose-dependent activity in E. coli and S. aureus, suggesting the bactericidal nature of AgNPs. The PTAgNPs exhibited dose-dependent toxicity in the A431 cell line, with an IC50 of 54.56 µg/mL arresting cell growth at the S phase, as revealed by flow cytometry analysis. The COMET assay revealed 39.9% and 18.15 severities of DNA damage and tail length in the treated cell line, respectively. Fluorescence staining studies indicate that PTAgNPs cause reactive oxygen species (ROS) and trigger apoptosis. This research demonstrates that synthesized silver nanoparticles have a significant effect on inhibiting the growth of melanoma cells and other forms of skin cancer. The results show that these particles can cause apoptosis or cell death in malignant tumor cells. This suggests that they could be used to treat skin cancers without harming normal tissues.