定量测量微小RNA（miRNA）在许多生物医学应用中特别是癌症诊断中非常重要，但仍然是一个巨大的挑战。在这项工作中，我们开发了一种基于“三位一体”分子识别模式的逻辑门识别生物感应平台，用于定量miRNA，检测限为4.48 aM，线性范围为0.1 nM到10 aM，在最佳实验条件下。为了获得出色的检测性能，我们采用了Zn（TCPP）光催化电子转移可逆加成-断裂链转移（PET-RAFT）聚合信号放大策略。光诱导的PET-RAFT已经在生物传感器领域中开发了自由基聚合的绿色应用。这是关于使用PET-RAFT制备信号放大生物传感器进行肿瘤标志物检测的第一篇报告。通过卓越的检测性能，我们可以将传感器系统应用于肺癌患者的早期筛查。

Quantitative measurement of microRNAs (miRNAs) is extremely important in plenty of biomedical applications especially cancer diagnosis but remains a great challenge. In this work, we developed a logic gate recognition biosensing platform based on the "trinity" molecular recognition mode for quantifying miRNAs with a detection limit of 4.48 aM, along with a linear range from 0.1 nM to 10 aM under optimal experimental conditions. In order to obtain excellent detection performance, we adopted a Zn(TCPP) photocatalytic electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization signal amplification strategy. The light-induced PET-RAFT has developed green applications of free radical polymerization in the field of biosensors. This is the first report on the preparation of signal amplification biosensors using PET-RAFT for tumor marker detection. With the outstanding detection performance, we can apply the sensor system to the early screening of lung cancer patients.