EUS精细针生物活检（FNB）针对淋巴结病变（LA）的作用仍在评估中。我们旨在评估EUS-FNB在诊断LA中的诊断准确性和不良事件率。从2015年6月到2022年，所有前往4个机构进行中隔和腹部LA EUS-FNB的患者全部纳入。使用22G Franseen尖或25G Fork尖针。阳性结果的金标准是手术或影像学及至少一年的临床观察。共纳入100例连续患者，其中包括新诊断的LA（40％），先前有肿瘤病史的LA（51％）或疑似淋巴增生疾病的患者（9％）。对于所有LA患者，EUS-FNB在2到3次穿刺（平均2.62±0.93）中技术可行。EUS-FNB的总体敏感性、阳性预测值（PPV）、特异性、阴性预测值（NPV）和准确性分别为96.20％、100％、100％、87.50％和97.00％。组织学分析在89％的病例中可行。细胞学评估在67％的标本中进行。22G或25G针的准确性之间没有统计学差异（P = 0.63）。关于淋巴增生疾病的亚分析显示，敏感性和准确性分别为89.29％和90.0％。未记录到任何并发症。EUS-FNB采用新型端切针可用于诊断LA，并且具有可靠和安全的方法。组织学核心的高质量和良好的组织量允许进行转移性LA的完整免疫组化分析和淋巴瘤的精确亚型划分。版权所有©2023美国胃肠镜协会。由Elsevier Inc.出版。保留所有权利。

The role of the newer EUS-fine needle biopsy (FNB) needles in lymphadenopathies (LA) is still under evaluation. We aimed to evaluate the diagnostic accuracy and the adverse event rate of EUS-FNB in diagnosing LA.From June 2015 to 2022, all patients referred to 4 institutions for EUS-FNB of mediastinal and abdominal LA were enrolled. 22G Franseen tip or 25G Fork tip needles were used. The gold standard for positive results was surgery or imaging and clinical evolution over a follow-up of at least one year.A total of 100 consecutive patients were enrolled, consisting of those with a new diagnosis of LA (40%), presence of LA with a previous history of neoplasia (51%), or suspected lymphoproliferative disease (9%). EUS-FNB was technically feasible in all LA patients with 2 to 3 passes (mean 2.62±0.93). The overall EUS-FNB sensitivity, positive predictive value (PPV), specificity, negative predictive value (NPV), and accuracy were 96.20%, 100%, 100%, 87.50%, and 97.00%, respectively. Histological analysis was feasible in 89% of cases. Cytological evaluation was performed in 67% of specimens. There was no statistical difference between the accuracy of the 22G or 25G needle (p=0.63). A sub-analysis on lymphoproliferative disease revealed a sensitivity and accuracy of 89.29% and 90.0%. No complications were recorded.EUS-FNB with new end-cutting needles is a valuable and safe method to diagnose LA. The high quality of histological cores and the good amount of tissue allowed a complete immunohistochemical analysis of metastatic LA and precise subtyping of the lymphomas.Copyright © 2023 American Society for Gastrointestinal Endoscopy. Published by Elsevier Inc. All rights reserved.