结直肠癌是2019年与癌症相关死亡的第二大原因。本文研究了含有acertannin的枫属植物对azoxymethane（AOM）/dextran酸钠（DDS）诱导的结直肠癌生长和结肠内白细胞介素（IL）-1β、单核细胞趋化蛋白（MCP）-1、IL-10和程序性细胞死亡-1（PD-1）水平的影响。肠道癌症的诱发是通过腹腔注射AOM（10 mg/kg）在第0和第27天进行的。小鼠在第7-14天、第32-33天和第35-38天可饮用1%（w/v）的DDS饮用水。Acertannin（30和100 mg/kg）在第1-16天口服，第16-26天停止，然后在第27-41天再次口服。使用相应的ELISA试剂盒测量细胞因子、趋化因子和PD-1的结肠水平。与未受Acertannin治疗的小鼠相比，受Acertannin治疗的小鼠瘤体数和面积分别减少了53.9％和63.1％。此外，IL-1β、MCP-1、IL-10和PD-1的结肠水平分别减少了57.3％、62.9％、62.8％和100％，而Cox-2、TOX/TOX2、PD-1和STAT3磷酸化阳性细胞数量分别减少了79.6％、77.9％、93.8％和100％。综上所述，Acertannin对AOM/DSS诱导的结直肠肿瘤生长的抑制作用似乎与结肠内IL-1β、MCP-1、IL-10和PD-1水平的降低有关，通过在肿瘤微环境中下调COX-2和TOX/TOX2的表达实现。版权所有©2023 Elsevier B.V.出版。

Colon cancer was the second leading cause of cancer-related death in 2019. We herein investigated the effects of acertannin containing Acer species on azoxymethane (AOM)/dextran sulfate sodium (DDS)-induced colon cancer growth and changes in the colonic levels of interleukin (IL)-1β, monocyte chemoattractant protein (MCP)-1, IL-10, and programmed cell death-1 (PD-1). Colorectal carcinogenesis was induced by an intraperitoneal injection of AOM (10 mg/kg) on days 0 and 27. Mice were given 1% (w/v) DSS drinking water ad libitum on days 7-14, 32-33, and 35-38. Acertannin (30 and 100 mg/kg) was orally administered on days 1-16, discontinued for 11 days (days 16-26), and then administered again on days 27-41. The colonic levels of cytokines, a chemokine, and PD-1 were measured using the respective ELISA kits. The number and area of tumors in mice treated with acertannin (100 mg/kg) decreased by 53.9 and 63.1%, respectively. Furthermore, the colonic levels of IL-1β, MCP-1, IL-10, and PD-1 showed reductions of 57.3, 62.9, 62.8, and 100%, respectively, while the numbers of cyclooxygenase-2 (COX-2)-, thymocyte selection-associated high mobility group box proteins (TOX)/TOX2-, PD-1-, and signal transducer and activator of transcription 3 (STAT3) phosphorylation-positive numbers decreased by 79.6, 77.9, 93.8, and 100%, respectively. In conclusion, the inhibitory effects of acertannin on AOM/DSS-induced colon tumor growth appear to be associated with reductions in the colonic levels of IL-1β, MCP-1, IL-10, and PD-1 through the down-regulated expression of COX-2 and TOX/TOX2 in the tumor microenvironment.Copyright © 2023. Published by Elsevier B.V.