N-糖基化是蛋白质修饰中最常见的类型之一，对正常生理过程起着至关重要的作用。然而，异常的N-糖基修饰与各种疾病的病理过程密切相关，包括恶性转化和肿瘤进展等过程。已知在不同阶段的肝癌发生过程中，相关糖蛋白的N-糖基构象被改变。表征肝癌患者糖基异质性和生物学功能将有助于更深入地理解肝损伤和肝癌发生的分子机制。在本文中，我们回顾了N-糖基化在肝癌发生中的作用，重点关注上皮-间质转化、细胞外基质变化和肿瘤微环境的形成。我们强调了N-糖基化在肝癌病理过程中的作用以及其在肝癌治疗或诊断中的潜在应用。© 2023年。作者（们）。

N-glycosylation is one of the most common types of protein modifications and it plays a vital role in normal physiological processes. However, aberrant N-glycan modifications are closely associated with the pathogenesis of diverse diseases, including processes such as malignant transformation and tumor progression. It is known that the N-glycan conformation of the associated glycoproteins is altered during different stages of hepatocarcinogenesis. Characterizing the heterogeneity and biological functions of glycans in liver cancer patients will facilitate a deeper understanding of the molecular mechanisms of liver injury and hepatocarcinogenesis. In this article, we review the role of N-glycosylation in hepatocarcinogenesis, focusing on epithelial-mesenchymal transition, extracellular matrix changes, and tumor microenvironment formation. We highlight the role of N-glycosylation in the pathogenesis of liver cancer and its potential applications in the treatment or diagnosis of liver cancer.© 2023. The Author(s).